In Vivo uptake of polyisobutyl cyanoacrylate nanoparticles by rat liver Kupffer, endothelial, and parenchymal cells

• Published in: Journal of pharmaceutical sciences 1984-07, Vol.73 (7), p.980-982
• Main Authors: Lenaerts, V., Nagelkerke, J. F., Van Berkel, T. J. C., Couvreur, P., Grislain, L., Roland, M., Speiser, P.
• Format: Article
• Language: English
• Subjects: and parenchymal cells; Animals; Biological and medical sciences; Bucrylate - analysis; Cyanoacrylates - analysis; Distribution-in vivo uptake of polyisobutyl cyanoacrylate nanoparticles; Distribution—in vivo uptake of polyisobutyl cyanoacrylate nanoparticles, rat liver Kupffer, endothelial, and parenchymal cells; endothelial; Endothelium - cytology; General pharmacology; in vivo uptake; Kinetics; Kupffer Cells - metabolism; Liver - cytology; Liver - metabolism; Medical sciences; Microspheres; Nanoparticles-polyisobutyl cyanoacrylate; Nanoparticles—polyisobutyl cyanoacrylate, in vivo uptake, rat liver Kupffer, endothelial, and parenchymal cells; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Polyisobutyl cyanoacrylate-in vivo uptake of nanoparticles; Polyisobutyl cyanoacrylate—in vivo uptake of nanoparticles, rat liver Kupffer, endothelial, and parenchymal cells; rat liver Kupffer; Rats; Rats, Inbred Strains
• ISSN: 0022-3549; 1520-6017
• DOI: 10.1002/jps.2600730730

Polyalkyl cyanoacrylate nanoparticles were previously developed as a biodegradable, ultrafine, solid drug carrier. Distribution studies in the rat showed an intense and rapid hepatic uptake. This liver accumulation appears to represent, to a certain extent, extracellularly bound nanoparticles. During liver perfusion, 15–20% of the liver‐associated nanoparticles were washed out. The cellular distribution of strongly cell‐associated nanoparticles was determined. At different intervals after injection of radioactive nanoparticles to rats, the cells were isolated according to a recently developed, low‐temperature procedure during which processing of the carrier was inhibited. At all tested times, a relatively intense capture by Kupffer cells in comparison with endothelial and especially parenchymal cells was observed. This distribution pattern was not influenced by the size of the nanoparticles (0.08–0.215‐μm diameter). This specific‐interaction of nanoparticles with Kupffer cells opens possibilities for the treatment of some parasitic diseases involving this cell type.