The role of glucose metabolism in a pig heart model of short-term hibernation

Previously, we reported, alterations in glucose metabolism in a 4 day model of chronic coronary stenosis similar to those described in patients with hibernating hearts. The purpose of this study was 2 fold: (1) to identify whether an acute model of mild, sustained ischemia could effect similar chang...

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Bibliographic Details
Published in:Molecular and cellular biochemistry 1998-03, Vol.180 (1-2), p.75-83
Main Authors: Hacker, T A, Renstrom, B, Nellis, S H, Liedtke, A J
Format: Article
Language:English
Subjects:
Animals
Fatty Acids - metabolism
Glucose - metabolism
Glycolysis - drug effects
Heart - physiology
Hibernation - physiology
Iodoacetates - pharmacology
Iodoacetic Acid
Ischemia
Metabolism
Models, Cardiovascular
Muscular system
Myocardial Contraction
Myocardium - metabolism
Oxygen Consumption
Space life sciences
Swine
Time Factors
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Summary:Previously, we reported, alterations in glucose metabolism in a 4 day model of chronic coronary stenosis similar to those described in patients with hibernating hearts. The purpose of this study was 2 fold: (1) to identify whether an acute model of mild, sustained ischemia could effect similar changes, and (2) to determine the effects of pharmacological inhibition of glycolysis. In the first group, extracorporeally perfused, intact pig hearts were subjected to 85 min of a 40% reduction in left anterior descending (LAD) coronary arterial blood flow. A second group was subjected to the same protocol, except after 40 min of LAD regional ischemia, iodoacetate (IAA) was administered to block glycolysis. Ischemia reduced MVO2 by 10% in both groups with a further 20% reduction noted following IAA treatment. Regional systolic shortening was reduced nearly 50% by ischemia and decreased an additional 40% following treatment with IAA. Glycolysis was increased by over 700% with ischemia in the first group. IAA caused a 3 fold reduction in glycolysis as compared to the preceding ischemic period and inhibited lactate production. Fatty acid metabolism was significantly reduced by ischemia in the first group, but was not reduced in the IAA group. Activity of creatine kinase associated with myofibrils was reduced and may have contributed to the contractile dysfunction. In conclusion, this acute model of short-term hibernation demonstrates several metabolic changes previously reported in chronic hibernation and may prove useful in determining mechanisms of substrate utilization in simulated conditions of chronic coronary stenosis and hibernation.
ISSN:0300-8177
1573-4919
DOI:10.1023/A:1006839022943