Sow vaccination modulates the colonization of piglets by Haemophilus parasuis

• Published in: Veterinary microbiology 2010-10, Vol.145 (3), p.315-320
• Main Authors: Cerdà-Cuéllar, M., Naranjo, J.F., Verge, A., Nofrarías, M., Cortey, M., Olvera, A., Segalés, J., Aragon, V.
• Format: Article
• Language: English
• Subjects: Animals; Animals, Newborn; Antibodies, Bacterial - blood; bacterial infections; Bacterial Vaccines - immunology; Bacterial Vaccines - pharmacology; Bacteriology; Biological and medical sciences; Cluster Analysis; Colonization; disease outbreaks; DNA Fingerprinting - methods; DNA Fingerprinting - veterinary; Female; Fundamental and applied biological sciences. Psychology; Genetic Variation; Genotype; H. parasuis; Haemophilus Infections - immunology; Haemophilus Infections - microbiology; Haemophilus Infections - prevention & control; Haemophilus Infections - veterinary; Haemophilus parasuis; Haemophilus parasuis - genetics; Haemophilus parasuis - immunology; Immunity, Maternally-Acquired - immunology; maternal immunity; microbial colonization; microbial genetics; Microbiology; Miscellaneous; molecular sequence data; nucleotide sequences; piglets; polymerase chain reaction; Polymerase Chain Reaction - veterinary; respiratory tract diseases; Respiratory Tract Diseases - immunology; Respiratory Tract Diseases - microbiology; Respiratory Tract Diseases - prevention & control; Respiratory Tract Diseases - veterinary; sows; strains; Swine; swine diseases; Swine Diseases - immunology; Swine Diseases - microbiology; Swine Diseases - prevention & control; Vaccination; Vaccination - methods; Vaccination - veterinary
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2010.04.002

Haemophilus parasuis is the etiologic agent of Glässer's disease in pigs and a colonizer of the upper respiratory tract of healthy pigs. A good balance between colonization and immunity is important to avoid a disease outbreak. This work studied the colonization of H. parasuis in healthy piglets coming from vaccinated and non-vaccinated sows. Piglets from vaccinated sows had higher IgG levels at early time points and subsequently were colonized later and to a lower degree than piglets from non-vaccinated ones. The variability of H. parasuis isolates was investigated by 2 genotyping methods: enterobacterial repetitive intergenic consensus (ERIC)-PCR and multilocus sequence typing (MLST). A high turnover of strains was found in both groups of piglets, with few strains found on more than one sampling occasion. We found a higher number of H. parasuis strains (16 strains) within a given farm than previously thought. Overall, more H. parasuis diversity was found in piglets from non-vaccinated sows than in those from vaccinated sows. These results indicate that vaccination of sows in a farm delays the colonization of piglets and reduces the carriage and heterogeneity of H. parasuis strains.