Mechanisms of Positive Effects of Transplantation of Human Placental Mesenchymal Stem Cells on Recovery of Rats after Experimental Ischemic Stroke

Mesenchymal stem cells isolated from human placenta and in vitro labeled with fluorescent magnetic microparticles were intravenously injected to rats 2 days after induction of focal cerebral ischemia (endovascular model). According to MRT findings, transplantation of mesenchymal stem cells led to an...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2009-12, Vol.148 (6), p.862-868
Main Authors: Yarygin, K. N, Kholodenko, I. V, Konieva, A. A, Burunova, V. V, Tairova, R. T, Gubsky, L. V, Cheglakov, I. B, Pirogov, Yu. A, Yarygin, V. N, Skvortsova, V. I
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Biophysics and Biochemistry
Brain Ischemia - therapy
Cell Biology
Cell Differentiation - physiology
Cell Proliferation
Cells, Cultured
differentiation
Disease Models, Animal
Female
Humans
Internal Medicine
ischemic stroke
Laboratory Medicine
Male
Mesenchymal Stem Cell Transplantation
mesenchymal stem cells
Mesenchymal Stem Cells - cytology
Mesenchymal Stem Cells - physiology
paracrine effect
Pathology
Placenta - cytology
Pregnancy
Rats
Stroke - therapy
substitute therapy
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Summary:Mesenchymal stem cells isolated from human placenta and in vitro labeled with fluorescent magnetic microparticles were intravenously injected to rats 2 days after induction of focal cerebral ischemia (endovascular model). According to MRT findings, transplantation of mesenchymal stem cells led to an appreciable reduction of the volume of ischemic focus in the brain. Two or three weeks after transplantation, labeled cells accumulated near and inside the ischemic focus, in the hippocampus, and in the subventricular zone of both hemispheres. Only few human mesenchymal stem cells populating the zone adjacent to the ischemic focus started expressing astroglial and neuronal markers. On the other hand, transplantation of mesenchymal stem cells stimulated proliferation of stem and progenitor cells in the subventricular zone and migration of these cells into the ischemic zone. Positive effects of transplantation of these cells to rats with experimental ischemic stroke are presumably explained by stimulation of proliferation of resident stem and progenitor cells of animal brain and their migration into the ischemic tissue and adjacent areas. Replacement of damaged rat neurons and glial cells by transplanted human cells, if it does take place, is quite negligible.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-010-0837-z