Energy substrate utilization in the common brushtailed possum ( Trichosurus vulpecula) using intravenous tolerance tests

• Published in: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2011-02, Vol.158 (2), p.132-135
• Main Authors: Legge, M., Jones, L.M., McLeod, B.J.
• Format: Article
• Language: English
• Subjects: 3-hydroxybutyric acid; 3-Hydroxybutyric Acid - metabolism; adults; Alanine; Alanine - metabolism; Animals; biomarkers; blood; blood glucose; Blood Glucose - metabolism; Brushtailed possum; energy; fasting; Female; females; Gluconeogenesis; Glucose; Glucose Tolerance Test; glucose tolerance tests; Injections, Intravenous; intravenous injection; Lactic Acid - metabolism; lipolysis; Male; males; possums; Propionate tolerance tests; Propionates - metabolism; propionic acid; Trichosurus - metabolism; Trichosurus vulpecula
• ISSN: 1096-4959; 1879-1107
• DOI: 10.1016/j.cbpb.2010.10.007

The aim of this study was to investigate the energy substrate requirements of the common brushtailed possum ( Trichosurus vulpecula) using intravenous tolerance tests for glucose, alanine, and propionate in five adult male and female animals under standardized conditions. Significant differences ( p < 0.01) were observed for fasting blood glucose values between males (6.3 ± 0.16 mmol L − 1 ) and females (4.8 ± 0.13 mmol L − 1 ), and males had a significantly ( p < 0.001) increased response to glucose. All animals returned to fasting glucose levels within 120 min after the glucose challenge. No significant change in blood glucose levels was observed for either the alanine or propionate tolerance tests ( p > 0.05). However, following propionate administration, there was a highly significant ( p < 0.001) decrease in blood lactate concentrations over 120 min. There was no evidence of ketone formation using β-hydroxybutyrate as a biomarker during any of the tests, indicating that there was no significant switch to lipolysis. In conclusion, the study provides new information on energy substrate utilization in this species and has identified that a gluconeogenic response normally identified in other species is not apparent in the common brushtailed possum.