Differential expression of genes in adipose tissue of first-lactation dairy cattle

• Published in: Journal of dairy science 2011, Vol.94 (1), p.361-369
• Main Authors: Sumner-Thomson, J.M, Vierck, J.L, McNamara, J.P
• Format: Article
• Language: English
• Subjects: adipose tissue; Adipose Tissue - metabolism; Animal Feed - analysis; Animal Nutritional Physiological Phenomena; Animal productions; Animals; Biological and medical sciences; Cattle - metabolism; Cattle - physiology; dairy cows; Diet - veterinary; early lactation; Fatty Acid Transport Proteins - genetics; fatty acid-binding proteins; Female; Food industries; Fundamental and applied biological sciences. Psychology; gene expression; Gene Expression Profiling - veterinary; Gene Expression Regulation; heifers; Holstein; Lactation - physiology; lipid metabolism; lipogenesis; Lipogenesis - genetics; lipolysis; Lipolysis - genetics; lipoprotein lipase; Lipoprotein Lipase - genetics; Milk and cheese industries. Ice creams; Pregnancy; RNA; Terrestrial animal productions; transcriptome; transport proteins; Vertebrates
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.2010-3447

Adipose tissue metabolism is an essential factor in establishment of a successful lactation, and we have a good understanding of changes in metabolic flux in relation to lactation, parity, and diet. However, the mechanisms of control of flux are less well understood. To continue our investigations into the control of adipose tissue metabolism, we conducted a transcriptomic analysis of adipose tissue of dairy cattle in late pregnancy and early lactation. Our objective was to determine the changes in gene expression in adipose tissue between 30 d prepartum and 14 d in milk in first-lactation animals, and to determine if changes in expression were related to practical production variables. Animals were Holstein heifers fed the same diet to National Research Council requirements, and adipose tissue was biopsied at 30 d prepartum and 14 DIM. Total RNA was extracted and used to determine gene expression on a bovine gene array. Genes that code for proteins controlling fatty acid transport were highly expressed including fatty acid binding proteins (FABP4 and FABP5) and lipoprotein lipase. Among those genes increasing in expression were those controlling lipolysis, including ADRB2 (52%) and LIPE (23%). Many genes coding for enzymes controlling lipogenesis decreased, including SREBP (−25%), TSHSP14 (−30.8%), LPL (−48.4%), and ACACA (−63.9%). This gene expression array analysis in adipose tissue of lactating dairy cattle identifies several key genes that are components of the adaptation to lactation that can be incorporated into models of nutritional efficiency and may be amenable to genetic or dietary manipulation.