Specificity Differentiation of Herpes Simplex Virus Types 1 and 2 by Indirect Immunofluorescence

Cells infected with herpesviruses develop nuclear and cytoplasmic antigens that are detectable by immunofluorescence. Specific antibodies to a type 1 and a type 2 herpes simplex virus were prepared in rabbits and goats, respectively. Reciprocal cross-titrations of these sera were done on antigens of...

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Bibliographic Details
Published in:The Journal of infectious diseases 1974-07, Vol.130 (1), p.63-66
Main Authors: Fraser, C. E. O., Melendez, L. V., Simeone, T.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies, Viral - isolation & purification
Antibody Specificity
Antigens
Antigens, Viral - isolation & purification
Antiserum
Cellular immunity
Epitopes
Fluorescent Antibody Technique
Genitalia
Goats
Herpesviridae
Herpesviridae - isolation & purification
Humans
Immune Sera - analysis
Indirect fluorescent antibody technique
Infections
Mathematics
Rabbits
Simplexvirus
Simplexvirus - classification
Simplexvirus - immunology
Viruses
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Summary:Cells infected with herpesviruses develop nuclear and cytoplasmic antigens that are detectable by immunofluorescence. Specific antibodies to a type 1 and a type 2 herpes simplex virus were prepared in rabbits and goats, respectively. Reciprocal cross-titrations of these sera were done on antigens of types 1 and 2 by the indirect immunofluorescence technique. The titers obtained were converted to index numbers and inserted into the formula for specificity differentiation. By this means 11 isolates were typed (seven type 1 and four type 2). Sera from patients with type 1 or 2 herpes simplex virus were titrated against viral antigens of types 1 and 2. The titers were applied to the II/I index formula to type the human sera. By these methods indirect immunofluorescence using intracellular antigens was shown to be a useful additional tool for typing herpes simplex viruses and sera of infected persons.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/130.1.63