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“Feeding the Baby”-Designing the Culture Milieu to Enhance Cell Stability
Attention was drawn to the responsiveness of cells, in vivo and in vitro, to small changes in their environment. Some of the more important, and the more often neglected, influences of the milieu on the genotypic and phenotypic stability and instability of cells in vitro were reviewed. Among the fac...
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| Published in: | JNCI : Journal of the National Cancer Institute 1974-11, Vol.53 (5), p.1443-1448 |
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| Format: | Article |
| Language: | English |
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| container_end_page | 1448 |
| container_issue | 5 |
| container_start_page | 1443 |
| container_title | JNCI : Journal of the National Cancer Institute |
| container_volume | 53 |
| creator | Waymouth, C |
| description | Attention was drawn to the responsiveness of cells, in vivo and in vitro, to small changes in their environment. Some of the more important, and the more often neglected, influences of the milieu on the genotypic and phenotypic stability and instability of cells in vitro were reviewed. Among the factors affecting one or more forms of cell stability were microorganisms (sometimes unrecognized), serum proteins, enzymes, cations, ionic balance and pH, O2 tension, hormones, and macromolecular growth .factors. It was suggestedthat high priority should be given to the design of defined milieus for enhancing the genotypic and phenotypic stability of specific cell types, and to the elimination of the hazards, variabilities, and uncertainties associated with the use of serum in culture media. |
| doi_str_mv | 10.1093/jnci/53.5.1443 |
| format | article |
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| fulltext | fulltext |
| identifier | ISSN: 0027-8874 |
| ispartof | JNCI : Journal of the National Cancer Institute, 1974-11, Vol.53 (5), p.1443-1448 |
| issn | 0027-8874 1460-2105 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_82565950 |
| source | Oxford University Press Archive |
| subjects | Adrenal Cortex Hormones Amino Acids Blood Calcium Cell Transformation, Neoplastic Cells, Cultured Culture Media Culture Techniques Genotype Growth Substances Hydrogen-Ion Concentration Insulin Membrane Potentials Oxygen Partial Pressure Phenotype Potassium Purines Pyrimidines Trace Elements Vitamins |
| title | “Feeding the Baby”-Designing the Culture Milieu to Enhance Cell Stability |
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