Effect of sodium cyanate upon the function of normal human polymorphonuclaer leukocytes

Sodium cyanate, a drug that prevents sickling of hemoglobin S by virtue of its irreversible carbamylation of the N-terminal amino group of valine, was studied for its effect upon the function of normal human polymorphonuclear luekocytes. In concentrations of 500 and 100 mug/ml, sodium cyanate was fo...

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Bibliographic Details
Published in:The Journal of infectious diseases 1975-05, Vol.131 Suppl, p.S73-S80
Main Authors: Ratzan, K R, Giraudo, C, Amado, C, Lauredo, I, Horowitz, G
Format: Article
Language:English
Subjects:
Blood Bactericidal Activity - drug effects
Carbon Dioxide - biosynthesis
Carbon Radioisotopes
Cyanates - pharmacology
Enterococcus faecalis - immunology
Escherichia coli - immunology
Glucose - metabolism
Humans
Iodine Radioisotopes
Isotope Labeling
Neutrophils - drug effects
Neutrophils - immunology
Neutrophils - metabolism
Oxidation-Reduction
Phagocytosis - drug effects
Sodium - pharmacology
Staphylococcus - immunology
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Summary:Sodium cyanate, a drug that prevents sickling of hemoglobin S by virtue of its irreversible carbamylation of the N-terminal amino group of valine, was studied for its effect upon the function of normal human polymorphonuclear luekocytes. In concentrations of 500 and 100 mug/ml, sodium cyanate was found to inhibit killing by neutrophils of Staphylococcus epidermidis and Eschierichia coli but not of Streptococcus faecalis. Viability of cells and phagocytosis were not affected by cyanate; however, production of [14-C] carbon dioxide from [1-14-C] glucose and the iodination of 125-I during phagocytosis were significantly impaired. Cyanate is thought to inbibit the bacterixidal activity of neutrophils by interfering with the oxidative metabolism of gluxose via the hexose monophosphate shunt (theraby decreasing production of H-2-O-2) and by inbibiting iodination of ingested bacteria (either by competing with iodide as the oxidizable cofactor or by inhibiting myeloperoxidase). Since these effects of cyanate were all reversible by washing the nurtrophils free of the drug, it is unlikely that they are due to amino carbamylation.
ISSN:0022-1899