Influence of antidiuretic hormone on release of lysosomal hydrolase at mucosal surface of epithelial cells from urinary bladder

THE permeability change induced by antidiuretic hormone (ADH) in amphibian urinary bladder is limited to the apical membrane of epithelial cells rich in intracellular secretion granules 1 . These granules, which may correspond to lysosomes 2 , are observed to migrate to and fuse with the apical memb...

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Bibliographic Details
Published in:Nature (London) 1975-10, Vol.257 (5526), p.493-495
Main Authors: PIETRAS, RICHARD J, SEELER, BARBARA J, SZEGO, CLARA M
Format: Article
Language:English
Subjects:
Acid Phosphatase - metabolism
Animals
Anura
Cathepsins - metabolism
Electric Conductivity
Epithelial Cells
Epithelium - enzymology
Glucuronidase - metabolism
Humanities and Social Sciences
Hydrocortisone - pharmacology
Hydrolases - metabolism
In Vitro Techniques
letter
Lysosomes - enzymology
Mucous Membrane - enzymology
multidisciplinary
Permeability
Rana catesbeiana
Science
Science (multidisciplinary)
Urinary Bladder - enzymology
Urinary Bladder - ultrastructure
Vasopressins - antagonists & inhibitors
Vasopressins - pharmacology
Water - metabolism
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Summary:THE permeability change induced by antidiuretic hormone (ADH) in amphibian urinary bladder is limited to the apical membrane of epithelial cells rich in intracellular secretion granules 1 . These granules, which may correspond to lysosomes 2 , are observed to migrate to and fuse with the apical membrane of toad bladder epithelia treated with oxytocin or cyclic AMP 3 . We have extended these observations and found that ADH elicits a marked, time-dependent increment in the release of lysosomal hydrolases into the extracellular medium of epithelial cells isolated from amphibian bladder. This effect is abolished by specific antiserum to ADH and reduced by preincubation of cells with cortisol. Additional experiments with intact bladder verify that the hydrolases so released occur predominantly at the mucosal surface. Moreover, cortisol antagonises the enhancement of transepithelial water transport and membrane conductance attributable to ADH action.
ISSN:0028-0836
1476-4687
DOI:10.1038/257493a0