Simultaneous HPLC analysis of triamcinolone acetonide and budesonide in microdialysate and rat plasma: Application to a pharmacokinetic study

A specific and reliable HPLC-PDA method for the quantitative determination of triamcinolone acetonide, budesonide and fluticasone propionate (as internal standards) in small volumes of microdialysate and rat plasma was developed. An efficient solid-phase extraction (SPE) procedure for plasma samples...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2010-11, Vol.878 (29), p.2967-2973
Main Authors: Vieira, Manuela de L.T., Singh, Rajandra P., Derendorf, Hartmut
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Animals
Anti-Inflammatory Agents - blood
Anti-Inflammatory Agents - pharmacokinetics
Biological and medical sciences
Blood
Budesonide
Budesonide - blood
Budesonide - pharmacokinetics
Chromatography
Chromatography, High Pressure Liquid - methods
Cleaning
Fundamental and applied biological sciences. Psychology
General pharmacology
HPLC
Male
Mathematical analysis
Matrices
Medical sciences
Microdialysis
Muscles
Muscles - chemistry
Pharmacology. Drug treatments
Rats
Rodents
Solid Phase Extraction
Tantalum
Triamcinolone acetonide
Triamcinolone Acetonide - blood
Triamcinolone Acetonide - pharmacokinetics
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Summary:A specific and reliable HPLC-PDA method for the quantitative determination of triamcinolone acetonide, budesonide and fluticasone propionate (as internal standards) in small volumes of microdialysate and rat plasma was developed. An efficient solid-phase extraction (SPE) procedure for plasma samples yielded extremely clean extracts with overall recovery of 104.3% and 95.7% for triamcinolone acetonide (TA) and fluticasone propionate, respectively. Plasma extracts obtained after SPE and microdialysis samples were directly injected on a C18 columm to separation. The method has been validated with good linearity, sensitivity, specificity and high accuracy (RE −5.28% to 9.14%) and precision (CV 0.50% to 6.62%) on both matrices. In stability studies, TA and budesonide were stable during storage and assay procedures. The method was applied to a pharmacokinetic study in rodents using microdialysis to determine protein unbound TA concentrations in blood and muscle.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2010.08.048