Effects of EGF and bFGF on irradiated parotid glands

Radiotherapy is common treatment for head-and-neck cancer, during which the salivary glands are often included within the radiation field. The most common side effect of this treatment is the development of oral dryness (xerostomia). This study considers the administration of epidermal growth factor...

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Bibliographic Details
Published in:Annals of biomedical engineering 2005-05, Vol.33 (5), p.685-695
Main Authors: Thula, Taili T, Schultz, Gregory, Tran-Son-Tay, Roger, Batich, Christopher
Format: Article
Language:English
Subjects:
Animals
Apoptosis - drug effects
Apoptosis - radiation effects
Biodegradation
Cells, Cultured
Delayed-Action Preparations - administration & dosage
Delayed-Action Preparations - chemistry
Diffusion
Epidermal Growth Factor - administration & dosage
Fibroblast Growth Factor 2 - administration & dosage
In Vitro Techniques
Irradiation
Parotid Gland - drug effects
Parotid Gland - pathology
Parotid Gland - physiopathology
Parotid Gland - radiation effects
Particle Size
Proteins
Radiation Injuries - pathology
Radiation Injuries - prevention & control
Radiation therapy
Radiation-Protective Agents - administration & dosage
Rats
Rats, Sprague-Dawley
Rodents
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Summary:Radiotherapy is common treatment for head-and-neck cancer, during which the salivary glands are often included within the radiation field. The most common side effect of this treatment is the development of oral dryness (xerostomia). This study considers the administration of epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF or FGF2) at physiological concentrations before and after irradiation in order to repair radiation-induced damage in salivary gland cells. As a preliminary examination of the efficacy of this approach we have characterized the effects of EGF and bFGF on the apoptotic response of 15-Gy irradiated rat salivary glands in vitro. Also, we have developed a controlled-release delivery system to effectively administer the growth factor to the gland since local delivery is essential to avoid unwanted protection of cancer cells. In vitro administration of bFGF prior to and immediately after irradiation partially protected (44%) the rat parotid gland. EGF did not show any significant radioprotective effect on parotid glands after a single 15-Gy irradiation dose. Encapsulation, storage and release of bFGF from biodegradable 50/50 PLGA microspheres did not affect the functionality of the growth factor in vitro.
ISSN:0090-6964
1573-9686
DOI:10.1007/s10956-005-1853-z