Histochemical Localization of Choline Oxidase in Rat Liver and Kidney

RAT liver is capable of oxidizing hydrolysed acetylcholine 1 . It was shown to be due to oxidation of choline, and betaine aldehyde is the oxidation product 2 . The oxidase system involved was found to consist of a dehydrogenase, choline dehydrogenase, cytochrome C and cytochrome oxidase 3 . Though...

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Bibliographic Details
Published in:Nature (London) 1963-12, Vol.200 (4912), p.1218-1219
Main Authors: GUHA, SIBAJYOTI, WEGMANN, RAYMOND
Format: Article
Language:English
Subjects:
Alcohol Oxidoreductases
Animals
Cats
Choline
Guinea Pigs
Histocytochemistry
Humanities and Social Sciences
Kidney
letter
Liver - enzymology
multidisciplinary
Old Medline
Oxidoreductases
Rabbits
Rats
Science
Science (multidisciplinary)
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Summary:RAT liver is capable of oxidizing hydrolysed acetylcholine 1 . It was shown to be due to oxidation of choline, and betaine aldehyde is the oxidation product 2 . The oxidase system involved was found to consist of a dehydrogenase, choline dehydrogenase, cytochrome C and cytochrome oxidase 3 . Though this enzyme system does not need diphosphopyridine nucleotide (DPN), yet it is sensitive to the action of amytal 4 . This oxidase system is also sensitive to antimycin A , cyanide and azide, but to a much lesser extent than that of succinic oxidase system 5 . These findings indicate that a respiratory chain identical to that of succinic oxidase may be involved in the hydrogen transfer from choline. According to some authors, flavin adenine dinucleotide (FAD) may be involved in this reaction 6 , but by others it plays no part at all 7 . Choline oxidase activity was studied biochemically using different hydrogen acceptors as ferricyanide, methylene blue, cytochrome C , 2,6-dichlorophenol indophenol 8 and triphenyl tetrazolium bromide 9 . But phenazine methosulphate was found to be the only reliable hydrogen acceptor for purified choline dehydrogenase 7 . This oxidase may play an important part in the transfer of labile methyl group to methionine 10 .
ISSN:0028-0836
1476-4687
DOI:10.1038/2001218a0