Polypeptide elongation factor 1 and the control of elongation rate in rat liver in vivo

THE development of rapid kinetic methods for measurement of elongation rate in protein synthesis in vivo , independent of initiation rate, has made possible the study of the control of elongation in relation to changes in overall protein synthesis 1–4 . In rat liver a 40% reduction in elongation rat...

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Bibliographic Details
Published in:Nature (London) 1976-12, Vol.264 (5588), p.804-806
Main Authors: NIELSEN, JENNIFER B. K, PLANT, PATRICIA W, HASCHEMEYER, AUDREY E. V
Format: Article
Language:English
Subjects:
Animals
Cell-Free System
Humanities and Social Sciences
Kinetics
letter
Liver - metabolism
Male
multidisciplinary
Peptide Chain Elongation, Translational - drug effects
Peptide Elongation Factors
Polyribosomes - metabolism
Rats
Science
Science (multidisciplinary)
Solubility
Thyroidectomy
Triiodothyronine - pharmacology
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Summary:THE development of rapid kinetic methods for measurement of elongation rate in protein synthesis in vivo , independent of initiation rate, has made possible the study of the control of elongation in relation to changes in overall protein synthesis 1–4 . In rat liver a 40% reduction in elongation rate (from about six to four amino acid residues per ribosome) is associated with thyroparathyroidectomy 2,4 ; the normal rate is restored by triiodothyronine injections 2 . The work reported here concerns the role of polypeptide elongation factor 1 (EF1), the factor responsible for aminoacyl-tRNA binding to ribosomes, in the control of elongation rate in this system. Several studies have suggested that EF1 may have regulatory significance in eukaryotic systems 5,8 ; however no direct correlation between EF1 activity and elongation rate in vivo has previously been described.
ISSN:0028-0836
1476-4687
DOI:10.1038/264804a0