Species Specificity of Cholecystokinin in Gut and Brain of Several Mammalian Species

Immunoreactive intact cholecystokinin and its COOH-terminal octapeptide are found in brain as well as in extracts of gut of the monkey, dog, and pig, by using an antiserum with equivalent sensitivities for detecting the octapeptide in free form or incorporated in the intact molecule. The failure to...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1978-01, Vol.75 (1), p.486-489
Main Authors: Straus, Eugene, Yalow, Rosalyn S.
Format: Article
Language:English
Subjects:
Animals
Antiserum
Brain
Brain Chemistry
Cerebral cortex
Cholecystokinin - analysis
Cholecystokinin - immunology
Cholecystokinin - metabolism
Digestion
Dogs
Gels
Haplorhini
Immunization
Immunoassay
Intestines - analysis
Molecules
Monkeys
Species Specificity
Swine
Trypsin - metabolism
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Description
Summary:Immunoreactive intact cholecystokinin and its COOH-terminal octapeptide are found in brain as well as in extracts of gut of the monkey, dog, and pig, by using an antiserum with equivalent sensitivities for detecting the octapeptide in free form or incorporated in the intact molecule. The failure to detect intact cholecystokinin in extracts from monkey or dog by using an antiserum developed by immunization with porcine cholecystokinin is presumed to be due to marked species differences in the NH2-terminal portion of the molecule. Tryptic digestion converted the intact cholecystokinin from all species to a peptide resembling the COOH-terminal octapeptide. The amount of cholecystokinin in the brain is comparable to that found in the gastrointestinal tract, the traditional site for this peptide.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.75.1.486