Naturally occurring respiratory deficient Candida slooffii strains resemble petite mutants

CYTOPLASMICALLY inherited respiratory deficient mutants termed petites , were first described in baker's yeast over 20 years ago 1 . Since then in laboratory studies several species of yeast have been shown to give rise to respiratory deficient mutants 2,3 . However, although some respiratory d...

Full description

Saved in:
Bibliographic Details
Published in:Nature (London) 1978-02, Vol.271 (5647), p.750-752
Main Authors: ARTHUR, HELEN, WATSON, K, McARTHUR, C. R, CLARK-WALKER, G. D
Format: Article
Language:English
Subjects:
Candida - genetics
Candida - metabolism
Chromosome Deletion
DNA, Circular - analysis
DNA, Mitochondrial - analysis
Humanities and Social Sciences
letter
multidisciplinary
Mutation
Oxygen Consumption
Science
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:CYTOPLASMICALLY inherited respiratory deficient mutants termed petites , were first described in baker's yeast over 20 years ago 1 . Since then in laboratory studies several species of yeast have been shown to give rise to respiratory deficient mutants 2,3 . However, although some respiratory deficient (obligatory fermentative) yeasts have been found in nature 3 , it is not clear whether these species are the result of cytoplasmic ( petite ) or chromosomal lesions 4 because no suitable genetic tests are available. Nevertheless it is known that petite mutants of both Saccharomyces cerevisiae 5 and Tornlopsis glabrata 6 have large deletions from their mitochrondrial DNA with each independent isolate having a unique circular DNA size profile which can be regarded as a ‘fingerprint’. By contrast chromosomal or segregational mutants have intact and functional mitochondrial DNA, as shown by genetic tests 3,7,8 . Therefore it seemed possible that the type of lesion in naturally occurring respiratory deficient species could be determined by circular DNA profile analysis. Using this method, we show here that three independently isolated strains of Candida slooffii resemble petite mutants.
ISSN:0028-0836
1476-4687
DOI:10.1038/271750a0