DNA as a target for anticancer compounds screening directly by resonance light scattering technique

• Published in: Analyst (London) 2010-10, Vol.135 (10), p.2653-2660
• Main Authors: Chen, Zhanguang, Peng, Yurui, Chen, Maohuai, Chen, Xi, Zhang, Guomin
• Format: Article
• Language: English
• Subjects: Analytical chemistry; Antineoplastic Agents - blood; Antineoplastic Agents - chemistry; Biological and medical sciences; Bleomycin - blood; Bleomycin - chemistry; Chemistry; Dactinomycin - blood; Dactinomycin - chemistry; DNA - chemistry; Doxorubicin - blood; Doxorubicin - chemistry; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; General, instrumentation; Hydrogen-Ion Concentration; Light; Molecular and cellular biology; Molecular genetics; Scattering, Radiation; Spectrometric and optical methods; Spectrometry, Fluorescence - methods; Temperature; Time Factors; Transcription. Transcription factor. Splicing. Rna processing
• ISSN: 0003-2654; 1364-5528
• DOI: 10.1039/c0an00386g

Anticancer drugs which selectively interact with DNA can change DNA's conformation and inhibit the duplication or transcription of DNA. An instrument-based assay for directly screening DNA-targeted anticancer drugs using resonance light scattering (RLS) technique with a common spectrofluorometer was proposed. To monitor the proposed screening method, the interactions between three anticarcinogens (Adriamycin (ADM), Bleomycin A (BLMA), Actinomycin D (ACTD)) and DNA were studied. The sequence of binding constants for the three anticarcinogens obtained from RLS spectra is: K(RLS) (ACTD, 9.43 × 10(5) L mol(-1)) > K(RLS) (ADM, 6.67 × 10(5) L mol(-1)) > K(RLS) (BLMA, 8.88 × 10(3) L mol(-1)) and of binding numbers is: N(RLS) (ACTD, 3.36 mmol g(-1)) < N(RLS) (ADM, 3.81 mmol g(-1)) < N(RLS) (BLMA, 57.44 mmol g(-1)). From the results we got the sequence of combination intensity between these three drugs and DNA as follows: ACTD-DNA > ADM-DNA > BLMA-DNA, which was completely consistent with drug activity. The conclusion indicated that the present method was direct, rapid, reliable and was another important innovation of the application of RLS technique.