Ultrastructure of Human Endometrial Epithelium in Monolayer Culture with and without Steroid Hormones

Colonies of cells of epithelioid appearance were identified in monolayer cultures grown up to 50 days from normal human endometrial cell suspensions obtained by a method designed to insure a maximum harvest of glandular cells. Groups of these cells were separated from stromal cells by means of cloni...

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Bibliographic Details
Published in:In Vitro 1977-06, Vol.13 (6), p.344-356
Main Authors: Theodore M. Liszczak, Richardson, George S., MacLaughlin, David T., Kornblith, Paul L.
Format: Article
Language:English
Subjects:
Cell growth
Cell lines
Culture Techniques
Cultured cells
Endometrium
Endometrium - drug effects
Endometrium - ultrastructure
Epithelial cells
Epithelioid cells
Epithelium - drug effects
Epithelium - ultrastructure
Estradiol - pharmacology
Female
Flasks
Glycocalyx
Humans
Organoids - ultrastructure
Progesterone - pharmacology
Stromal cells
Tissue culture techniques
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Summary:Colonies of cells of epithelioid appearance were identified in monolayer cultures grown up to 50 days from normal human endometrial cell suspensions obtained by a method designed to insure a maximum harvest of glandular cells. Groups of these cells were separated from stromal cells by means of cloning cylinders. Studies comparing the ultrastructure of cells of this type to fresh endometrial tissue revealed a number of similarities. The morphological characteristics common to both types of samples included junctional complexes, perinuclear microfilaments and microvilli with glycocalyx. Other common features were prominent nucleoli, well developed Golgi, rough endoplasmic reticulum and membrane-bound electron-dense bodies in the cytoplasm. A stripping technique applied to the fetal bovine serum used in the nutrient medium made it possible to initiate cultures in a steroid-free environment and to maintain them in the presence of the specified concentration of estradiol and/or progesterone. Isolation of epithelial cells of endometrium in monolayer culture may provide a useful model system in which to study the specific effects of steroid hormones on cellular function and differentiation.
ISSN:0073-5655
1475-2689
DOI:10.1007/BF02615094