Treatment of Hypoplastic Anemia in Mice With Placental Transplants

A genetic mutation in mice (W/Wv) causes an autosomal recessive disease characterized by hypoplastic anemia which lasts throughout life. Double-dominant W/Wv anemic mice were sublethally irradiated to facilitate repopulation of marrow with transplanted cells and were injected intravenously with susp...

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Bibliographic Details
Published in:Blood 1977-10, Vol.50 (4), p.663-670
Main Authors: Dancis, Joseph, Jansen, Valerie, Brown, George F., Gorstein, Fred, Balis, M. Earl
Format: Article
Language:English
Subjects:
Aging
Anemia, Aplastic - genetics
Anemia, Aplastic - mortality
Anemia, Aplastic - therapy
Animals
Blood Transfusion
Electrophoresis, Cellulose Acetate
Erythropoiesis
Female
Fetus
Male
Mice
Mice, Inbred C57BL
Placenta - transplantation
Pregnancy
Radiation Chimera
Transplantation, Isogeneic
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Summary:A genetic mutation in mice (W/Wv) causes an autosomal recessive disease characterized by hypoplastic anemia which lasts throughout life. Double-dominant W/Wv anemic mice were sublethally irradiated to facilitate repopulation of marrow with transplanted cells and were injected intravenously with suspensions of 5-10 million placental cells of 15 days gestation derived from normal, isogeneic donors. Red cell counts fell promptly after irradiation and then rose progressively over a period of weeks, reaching normal levels for the nonmutant. Mean corpuscular volume and hemoglobin electrophoresis patterns of red cells in recipient W/Wv mice resembled those of normal donor animals. The therapeutic effect lasted for the duration of the observation period, in some instances over 9 mo. W/Wv mice that were administered Hanks’ solution or fetal blood, instead of placental transplants, remained anemic. Late gestation placentas (18 days) were also ineffective.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V50.4.663.663