Interaction of Salmonella with Phagocytes in Vitro

Cultures of macrophages obtained from normal mice had great ability to kill large numbers of Salmonella typhosa and virulent and avirulent strains of Salmonella typhimurium immediately after ingestion of these bacteria. The Ty2 strain of S. typhosa, which contains the Vi antigen, required the presen...

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Bibliographic Details
Published in:The Journal of infectious diseases 1965-04, Vol.115 (2), p.131-141
Main Authors: Morello, Josephine A., Baker, Edgar E.
Format: Article
Language:English
Subjects:
Animals
Antibiotics
Antibodies
Antiserum
Bacteria
Bacteriological Techniques
Cell culture techniques
Cultured cells
Immunization
In Vitro Techniques
Inoculum
Macrophages
Mice
Old Medline
Phagocytes
Phagocytosis
Pharmacology
Salmonella
Salmonella typhi
Salmonella typhimurium
Streptomycin
Tissue Culture Techniques
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Summary:Cultures of macrophages obtained from normal mice had great ability to kill large numbers of Salmonella typhosa and virulent and avirulent strains of Salmonella typhimurium immediately after ingestion of these bacteria. The Ty2 strain of S. typhosa, which contains the Vi antigen, required the presence of specific antibody in the tissue culture medium to be phagocytized to any great extent. Once ingested, however, the macrophages killed Ty2 in a manner similar to that of the other salmonellae tested. Cells obtained from immunized mice behaved like cells obtained from nonimmunized mice with respect to phagocytosis and killing of S. typhosa strain Ty2. Quantitation of the bactericidal rate of macrophages by comparison of the number of viable organisms released from the cells and the number of bacilli seen in macrophages on stained cover slip preparations was preferable to techniques which employed only one or the other of these methods because it permitted a better evaluation of the events which occurred in infected cell cultures. In preliminary experiments the presence of streptomycin adversely affected the outcome of cell culture studies. It therefore seems preferable to omit this antibiotic from such studies.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/115.2.131