Purification and Specific Kinetic Properties of Erythrocyte Uridine Diphosphate Glucose Pyrophosphorylase

The enzyme uridine diphosphate glucose pyrophosphorylase has been prepared at high purity (specific activity 127) from the human erythrocyte. Comparison of its specific properties with enzyme isolated from liver indicates many similarities including size. Examination of the reversible reaction catal...

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Bibliographic Details
Published in:The Journal of biological chemistry 1969-02, Vol.244 (4), p.1008-1015
Main Authors: Tsuboi, K K, Fukunaga, K, Petricciani, J C
Format: Article
Language:English
Subjects:
Chromatography, Gel
Chromatography, Ion Exchange
Erythrocytes - enzymology
Glucose
Humans
Kinetics
Liver - enzymology
Nucleotidyltransferases - isolation & purification
Nucleotidyltransferases - metabolism
Uracil Nucleotides
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Summary:The enzyme uridine diphosphate glucose pyrophosphorylase has been prepared at high purity (specific activity 127) from the human erythrocyte. Comparison of its specific properties with enzyme isolated from liver indicates many similarities including size. Examination of the reversible reaction catalyzed by the erythrocyte enzyme from both forward and reverse directions revealed a highly selective product inhibition by UDP-glucose. Since a dual enzyme function involving both regulation and synthesis of UDP-glucose was implied, additional UDP-glucose pyrophosphorylases were examined in relation to their product inhibition characteristics for comparative purposes. The product inhibition studies were extended and applied in distinguishing the kinetic mechanism of erythrocyte UDP-glucose pyrophosphorylase. From the product inhibition patterns and initial velocity studies an ordered Bi-Bi reaction mechanism was indicated in which nucleotide adds first as substrate to the enzyme and is last to be released. Michaelis constants for each of the substrates and dissociation (inhibition) constants for substrates combining with free enzyme were also determined.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)91886-7