Comparison of the chemical profiles and anti-platelet aggregation effects of two “Dragon's Blood” drugs used in traditional Chinese medicine

• Published in: Journal of ethnopharmacology 2011-01, Vol.133 (2), p.796-802
• Main Authors: Yi, Tao, Chen, Hu-Biao, Zhao, Zhong-Zhen, Yu, Zhi-Ling, Jiang, Zhi-Hong
• Format: Article
• Language: English
• Subjects: animal models; Animals; Anti-platelet aggregation; anticoagulant activity; antiplatelet aggregation activity; Arecaceae - chemistry; Biological and medical sciences; blood flow; chemical constituents of plants; Chemical profiles; China; Daemonorops; Daemonorops draco; dosage; Dracaena; Dracaena - chemistry; Dracaena cochinchinensis; Dragon's Blood; Drugs, Chinese Herbal - chemistry; Drugs, Chinese Herbal - pharmacology; Ethnopharmacology; flavanes; General pharmacology; herbal medicines; high performance liquid chromatography; In Vitro Techniques; Male; mass spectrometry; mechanism of action; Medical sciences; medicinal plants; Medicine, Chinese Traditional; Oriental traditional medicine; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; phytochemicals; Plant Extracts - chemistry; Plant Extracts - pharmacology; platelet activation; platelet aggregation; Platelet Aggregation - drug effects; Platelet Aggregation Inhibitors - chemistry; Platelet Aggregation Inhibitors - pharmacology; Rats; Rats, Sprague-Dawley; resins; Resins, Plant - chemistry; Resins, Plant - pharmacology; species differences; Spectrometry, Mass, Electrospray Ionization; stilbenes
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2010.11.008

The present study compared the chemical profiles and anti-platelet aggregation effects of two species of “Dragon's Blood”. The results demonstrated that the chemical profiles and pharmacological potencies of the two species were significantly different, even though their appearance looks similar. “Dragon's Blood” has been used as a medicine since ancient times by many cultures. In traditional Chinese medicine, the resin obtained from Daemonorops draco (RDD) and the resin from Dracaena cochinchinensis (RDC) are equally prescribed as “Dragon's Blood” for facilitating blood circulation. To verify the traditional efficacy and elucidate the mechanism, the present study compared the chemical profiles and the pharmacological effects of two species of “Dragon's Blood” mainly used in China. A UPLC-MS fingerprinting method was developed to compare the chemical profiles of the two medicines. The anti-platelet aggregation effects of the two medicines induced by arachidonic acid (AA) were investigated. The chemical profiles of these two species of “Dragon's Blood” were significantly different. The characteristic constituents were found to be: flavanes in RDD and stilbenes in RDC. In the in vivo platelet inhibition test, performed with the dose of 200mg/kg on rats, the peak inhibitory effects of RDD and RDC were 35.8% and 27.6%, respectively, compared with the control group. With the in vitro concentrations of 0.2, 0.4 and 0.8mg/ml, RDD exerted significant inhibition of aggregation by 18.7%, 20.0%, and 61.6%, respectively, and RDC exerted significant inhibition of aggregation by 13.3%, 20.2%, and 31.6%, respectively. The fingerprinting method used here is suitable for distinguishing them. All pharmacological tests indicated that RDD was more potent than RDC against platelet aggregation.