Effect of Deoxyribonucleic Acid and Histone on the Nuclear Ribosomal Incorporation System

IT is generally recognized that the role of DNA in protein synthesis is to provide a template for the transcription of messenger RNA which in turn translates the genetic code into amino-acid sequences. Holland and McCarthy 1–3 and Naora 4,5 have reported a direct stimulation of labelled amino-acid i...

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Bibliographic Details
Published in:Nature (London) 1966-11, Vol.212 (5065), p.928-930
Main Author: WANG, TUNG YUE
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Animals
Cattle
Cell Nucleus - drug effects
Cell Nucleus - metabolism
Cytosine Nucleotides - metabolism
Dactinomycin - pharmacology
DNA - pharmacology
Guanine Nucleotides - metabolism
Histones - pharmacology
Hot Temperature
Humanities and Social Sciences
In Vitro Techniques
letter
Leucine - metabolism
multidisciplinary
Nucleic Acid Denaturation
Protein Biosynthesis
Puromycin - pharmacology
Pyruvate Kinase - metabolism
Pyruvates - metabolism
Ribosomes - drug effects
Ribosomes - metabolism
Science
Science (multidisciplinary)
Stimulation, Chemical
Thymus Gland - cytology
Thymus Gland - metabolism
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Summary:IT is generally recognized that the role of DNA in protein synthesis is to provide a template for the transcription of messenger RNA which in turn translates the genetic code into amino-acid sequences. Holland and McCarthy 1–3 and Naora 4,5 have reported a direct stimulation of labelled amino-acid incorporation in vitro by denatured DNA with ribosomal systems of E. coli and calf thymus nuclei. This stimulatory effect on amino-acid incorporation by DNA does not involve enhanced RNA synthesis 1,2,4 and is apparently catalysed by the p H 5 fraction (ref. 4). Such an effect implies that single stranded DNA may participate in the processes of protein synthesis as a direct template and messenger. In order to investigate this phenomenon further and to test the possible effect of histone on the ribosomal incorporation system, investigations were carried out using materials prepared from the calf thymus. This report presents the results showing that the ammo-acid incorporation of calf thymus ribosomal system is markedly stimulated by native but not by heat denatured DNA, and is inhibited by histone, particularly the lysine-rich histone fraction.
ISSN:0028-0836
1476-4687
DOI:10.1038/212928a0