Isoelectric Focusing in Polyacrylamide Gel and its Application to Immunoglobulins

THE principle of isoelectric fractionation was applied to the separation of amino-acids as early as 1912 by Ikeda and Suzuki (Japanese patent quoted in ref. 8), but the technique did not become practical for the fractionation of macromolecular ampholytes such as proteins until the introduction of na...

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Bibliographic Details
Published in:Nature (London) 1968-07, Vol.219 (5149), p.66-67
Main Authors: AWDEH, Z. L, WILLIAMSON, A. R, ASKONAS, BRIGITTE A
Format: Article
Language:English
Subjects:
Acrylic Resins
Animals
Blood Protein Electrophoresis
Cetacea
Electrophoresis, Disc
gamma-Globulins - analysis
Gels
Hemoglobins - analysis
Humanities and Social Sciences
Hydrogen-Ion Concentration
letter
Mice
multidisciplinary
Myoglobin - analysis
Neoplasm Proteins - analysis
Rabbits
Science
Science (multidisciplinary)
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Description
Summary:THE principle of isoelectric fractionation was applied to the separation of amino-acids as early as 1912 by Ikeda and Suzuki (Japanese patent quoted in ref. 8), but the technique did not become practical for the fractionation of macromolecular ampholytes such as proteins until the introduction of natural p H gradients 1 and suitable carrier ampholytes 2–4 . Svensson described an apparatus for isoelectric focusing in shallow p H gradients formed by low molecular weight ampholytes and stabilized by sucrose density gradients 3 . In this form the technique has been successfully applied to studies on the heterogeneity of myoglobins 4,5 , cytochrome c 6 , lactoperoxidase 7 and a number of other proteins (see review by Haglund 8 ).
ISSN:0028-0836
1476-4687
DOI:10.1038/219066a0