Use of Light-scattering to Measure the Selective Interaction of a Macromolecular Solute with the Components of a Binary Solvent

A SOLUTION of a non-diffusible solute will in general contain the components of a mixed solvent in proportions different from those of an equilibrium, diffusate, owing to non-ideal interactions between, the solute and the solvent components. The distribution can be measured refractometrically 1,2 an...

Full description

Saved in:
Bibliographic Details
Published in:Nature (London) 1965-07, Vol.207 (4992), p.80-81
Main Authors: OGSTON, A. G, PRESTON, B. N
Format: Article
Language:English
Subjects:
Chemical Phenomena
Chemistry, Physical
Humanities and Social Sciences
In Vitro Techniques
letter
Light
Macromolecular Substances
multidisciplinary
Science
Solutions
Solvents
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A SOLUTION of a non-diffusible solute will in general contain the components of a mixed solvent in proportions different from those of an equilibrium, diffusate, owing to non-ideal interactions between, the solute and the solvent components. The distribution can be measured refractometrically 1,2 and can be expressed unambiguously for a binary solvent as the excess quantity of one of the solvent components (arbitrarily chosen) relative to unit quantity of the other, per unit quantity of solute. Direct refracto-metric measurement requires preparation of an equilibrium diffusate, and is limited with respect to the concentration of the solute by the sensitivity of the measurement. The use of diffusate can be avoided by measurement of light-scattering, which also allows measurements to be extrapolated reliably to zero concentration of solute.
ISSN:0028-0836
1476-4687
DOI:10.1038/207080a0