Induction of Photohemolysis by Tetrachlorosalicylanilide

The antibacterial agent 3,3′,4′,5-tetrachlorosalicylanilide (TCSA) enhanced the sensitivity of erythrocytes to long-wave UV light in vitro. The exposure of TCSA-treated red cells to UV radiation resulted in photohemolysis; the extent of photohemolysis was related to the duration of irradiation. The...

Full description

Saved in:
Bibliographic Details
Published in:Journal of pharmaceutical sciences 1968-12, Vol.57 (12), p.2136-2139
Main Authors: Oleniacz, W.S., Singer, E.J., Doyle, A.B., Vinson, L.J.
Format: Article
Language:English
Subjects:
Anilides
Animals
cations-UV light effect
Cell Membrane Permeability - drug effects
Cell Membrane Permeability - radiation effects
Cell permeability
Cell permeability, cations—UV light effect
Erythrocytes-photohemolysis determination
Hemolysis - radiation effects
Imidazoles
Light
Magnesium
Photohemolysis induction-tetrachlorosalicylanilide
Potassium
Rats
Sodium
Sulfonic Acids
Time Factors
Ultraviolet Rays
UV irradiation-photohemolysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The antibacterial agent 3,3′,4′,5-tetrachlorosalicylanilide (TCSA) enhanced the sensitivity of erythrocytes to long-wave UV light in vitro. The exposure of TCSA-treated red cells to UV radiation resulted in photohemolysis; the extent of photohemolysis was related to the duration of irradiation. The photohemolytic reaction appeared to be a consequence of UV excitation of TCSA to the triplet state and subsequent reaction of the latter molecule with the erythrocyte membrane. The interaction of the excited TCSA molecule with the red cell membrane was manifested as increased permeability towards Mg2+, K+, or Na+ ions. Photohemolysis was prevented by the UV absorbers urocanic acid and 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid. In addition to TCSA, 3,3′,4′,5- and 2′,3,4′,5-tetrabromosalicylanilides caused photohemolysis. Monobromo-, dibromo-, and tribromosalicylanilides failed to induce significant photohemolysis. The results suggest that some instances of photodermatitis due to TCSA may be related to changes in cell permeability and lysis.
ISSN:0022-3549
1520-6017
DOI:10.1002/jps.2600571223