Gene positioning is regulated by phosphorylation of the nuclear pore complex by Cdk1

In yeast, many genes are targeted to the nuclear periphery through interaction with the Nuclear Pore Complex upon activation. Targeting requires nucleoporin proteins and DNA elements in the promoters of these genes. We have recently found that targeting is regulated through the cell cycle. Immediate...

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Bibliographic Details
Published in:Cell cycle (Georgetown, Tex.) Tex.), 2011-02, Vol.10 (3), p.392-395
Main Authors: Garvey Brickner, Donna, Brickner, Jason H.
Format: Article
Language:English
Subjects:
Binding
Biology
Biomarkers - analysis
Bioscience
Calcium
Cancer
CDC2 Protein Kinase - physiology
Cell
Cell Cycle
Cycle
Extra View
Landes
Models, Genetic
Nuclear Envelope - ultrastructure
Nuclear Pore - metabolism
Nuclear Pore Complex Proteins - genetics
Nuclear Pore Complex Proteins - metabolism
Nuclear Pore Complex Proteins - physiology
Organogenesis
Phosphorylation
Proteins
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae - ultrastructure
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Saccharomyces cerevisiae Proteins - physiology
Telomere - genetics
Telomere - metabolism
Transcriptional Activation
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Summary:In yeast, many genes are targeted to the nuclear periphery through interaction with the Nuclear Pore Complex upon activation. Targeting requires nucleoporin proteins and DNA elements in the promoters of these genes. We have recently found that targeting is regulated through the cell cycle. Immediately following the initiation of DNA replication, active genes lose peripheral localization for ~30 minutes. This regulation is mediated by cyclic phosphorylation of a nucleoporin by Cdk1. Some genes that are targeted to the nuclear periphery upon activation remain at the nuclear periphery after repression, a phenomenon called transcriptional memory. Curiously, the mechanism that regulates localization of active genes to the nuclear periphery does not regulate the localization of the same genes after repression, suggesting that these genes are targeted by two distinct mechanisms. Finally, the localization of other parts of the genome that localize at the nuclear periphery seem to be regulated by a distinct mechanism, suggesting that the spatial organization of the genome is tightly coupled to the progression of the cell cycle.
ISSN:1538-4101
1551-4005
DOI:10.4161/cc.10.3.14644