Bovine Colostrum: Determination of Naturally Occurring Steroid Hormones by Liquid Chromatography−Tandem Mass Spectrometry (LC−MS/MS)

The aim of this study was to collect further data about levels of endogenous hormones in foodstuffs derived from animal production. Because of expected higher concentrations of especially estrogens in colostrum compared to other foodstuffs, our investigation focused on this matrix. For evaluation of...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2011-02, Vol.59 (4), p.1423-1427
Main Authors: Farke, Carolin, Rattenberger, Ernst, Roiger, Sabine U, Meyer, Heinrich H. D
Format: Article
Language:English
Subjects:
androgens
Androgens - analysis
animal production
Animals
Biological and medical sciences
Cattle - metabolism
Chromatography, Liquid
Colostrum - chemistry
conjugated estrogens
cow colostrum
estriol
Estrogens, Conjugated (USP) - analysis
estrone
Fats - analysis
Female
Food Chemistry/Biochemistry
Food industries
foods
Fundamental and applied biological sciences. Psychology
Hormones - analysis
liquid chromatography
mass spectrometry
milk
Milk - chemistry
milk fat
progesterone
Progesterone - analysis
steroid hormones
Tandem Mass Spectrometry
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Summary:The aim of this study was to collect further data about levels of endogenous hormones in foodstuffs derived from animal production. Because of expected higher concentrations of especially estrogens in colostrum compared to other foodstuffs, our investigation focused on this matrix. For evaluation of endogenous steroid hormones in separated colostrum (fat and defatted fraction) and colostrum powder, the relevant free and conjugated estrogens (estradiol-17β, estradiol-17α, estrone, and estriol) androgens (androstendione, 19nor-androstendione, 19nor-testosterone-17β, 19nor-testosterone-17α, testosterone-17β, and testosterone-17α), and progesterone were determined by means of liquid chromatography−tandem mass spectrometry (LC−MS/MS). Upmost determined concentrations were found in the fat fraction, with 25.56 and 7.59 μg/L for estrone and androstendione, respectively. In defatted milk and colostrum powder, conjugated estrogens dominated, whereas total (free and conjugated) estrone (5.51 μg/L; 15.0 μg/kg) exceeded estradiol-17α (2.66 μg/L; 7.5 μg/kg) and estradiol-17β (2.28 μg/L; 3.3 μg/kg). Neither 19nor-steroids nor estriol were detected in colostrum fractions or processed colostrum.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf103751z