Heparinized polyvinyl alcohol to specifically adsorb low-density lipoprotein from plasma

• Published in: Transfusion and apheresis science 2011-02, Vol.44 (1), p.3-9
• Main Authors: Ma, Kai-wang, Dai, Xiao-zhen, Feng, Shu-ying, Jing, Ai-hua, Yang, Jian-ying
• Format: Article
• Language: English
• Subjects: Adsorption; Blood Platelets - chemistry; Blood Platelets - drug effects; Blood Platelets - ultrastructure; Blood purification; Drug Stability; Health technology assessment; Hematology, Oncology and Palliative Medicine; Hemolysis; Heparin; Heparin - analogs & derivatives; Heparin - chemistry; Heparin - pharmacology; Humans; Hyperlipidemias - blood; Hyperlipidemias - therapy; LDL; Lipoproteins, LDL - blood; Lipoproteins, LDL - isolation & purification; Low-density lipoprotein; Materials Testing; Microscopy, Electron, Scanning; P-Selectin - analysis; Plasmapheresis - instrumentation; Plasmapheresis - methods; Platelet Activation - drug effects; Polyvinyl Alcohol - analogs & derivatives; Polyvinyl Alcohol - chemistry; Polyvinyl Alcohol - pharmacology; Spectrophotometry, Infrared
• ISSN: 1473-0502; 1878-1683
• DOI: 10.1016/j.transci.2010.12.001

Abstract Introduction A medical adsorbent for blood purification was developed to specifically adsorb low-density lipoprotein (LDL) from hypercholesterolemia patient’s plasma by covalently immobilizing heparin onto the surface of polyvinyl alcohol (PVA) with the couplant toluence-2,4-diisocyanate (TDI). Methods We used IR to demonstrate the success of covalently immobilizing heparin onto the surface, and investigated its adsorption of LDL, and primarily evaluated its hemo-compatibility using tests for platelet adhesion, the degree of platelet activation and a hemolysis test. Results (1) Heparin was successfully covalently immobilized onto the surface, the maximum amount of heparin immobilized on the surface of 1 g PVA–1799 granules was about 5 μg; (2) one optimal condition for adsorption of LDL from hyperlipidemia plasma was a pH within the range of 7.2∼9.5, accordingly the adsorptive ratio (adsorbent/g: plasma/L = 1:2) for LDL was about 70%; (3) it exhibited good hemo-compatibility. Conclusion The adsorbent results in satisfactory adsorption of LDL with good hemo-compatibility; it could potentially be used as a blood purification material, and immobilization of heparin onto medical materials may be a way to develop an LDL-specific adsorbent for blood purification.