Mapping the bovine factor H gene to chromosome 16 by SSCP analysis

• Published in: Mammalian genome 1997-01, Vol.8 (1), p.77-78
• Main Authors: Williams, J L, Lester, D H, Teres, V M, Barendse, W, Sim, R B, Soames, C J
• Format: Article
• Language: English
• Subjects: Alleles; Animals; Bos taurus; Cattle - genetics; Complement Factor H - genetics; Molecular Sequence Data; Polymerase Chain Reaction; Polymorphism, Single-Stranded Conformational
• ISSN: 0938-8990; 1432-1777
• DOI: 10.1007/s003359900360

Species: Bos taurus Locus name: Factor H Locus symbol: FH Map position: At the centromeric end of bovine Chromosome (Chr) 16; polyimmunoglobulin receptor 0cM (PIGR, lod score z = 8.43, theta = 0.0), BM6430 0cM (microsatellite marker, lod score z = 6.62, theta = 0.0), TGLA245 5cM (microsatellite marker, lod score z = 21.71, theta = 0.5), XGTG1 9cM (microsatellite marker, lod score z = 10.41; theta = 0.09) and ATP dependent calcium pump 11cM (ATPCP lod score z = 5.18, theta = 0.11). Method of mapping: International bovine reference panel. Database deposit information: Mapping information available through the cattle genotype database (Barendse). FH sequence data from EMBL, Accession number X98697. Allele detection: by single-stranded conformational polymorphism (SSCP). Approximately 2000 base pairs of bovine FH sequence were compared with the sequences of murine and human FH (Accession numbers M29009 and P08603 respectively). From the known exonic and intronic structure of the murine and human complement factor H it was possible to predict the putative exon intron boundaries in the bovine factor H gene. PCR primers were designed to amplify from exon 8 across intron 8 and into exon 9 of bovine factor H. This intron is one of the smaller introns in the murine and human factor H gene. Oligonucleotide primers: Bovine exon 8 forward 5'-CCTCCTTCACAACGTTCCTGGGACC-3' Bovine exon 9 reverse 5'-GCAGTGAACTCTTACAGTTTCACCC-3'. Discussion: This linkage result confirms the previous mapping of factor H to bovine Chr 16 with somatic cell hybrids and localizes the gene near the centromere. Factor H, complement receptors 1 and 2 (CR1 and CR2), C4 binding protein, decay-accelerating factor (DAF), and membrane cofactor protein (MCP) can all be considered to be part of the same supergene family, the regulators of complement activation (RCA) family, which is clustered on the long arm of human Chr 1 (1q32.1). RCA proteins share several structural and functional characteristics; for example, all have repeating homology units (named CCPs or SCRs) of approximately 60 residues. CR2 has previously been shown to map to human Chr 1, bovine Chr 16, and mouse Chr 1, while chromosome painting suggests that synteny is conserved between bovine Chr 16 and human Chr 1. The localization of FH near the centromere of bovine Chr 16 supports this conservation of synteny, and it is likely that other bovine RCA proteins will also map in this region.