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A new approach to examining scorpion peg sensilla: the mineral oil flood technique

All scorpions possess jointed, ventral appendages called pectines. These organs have chemosensory, peg-shaped sensilla that detect substrate-borne chemicals. Previous physiological studies show that neurons within peg sensilla respond to an assortment of volatile organic chemical stimulants blown ac...

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Bibliographic Details
Published in:The Journal of arachnology 2009-01, Vol.37 (3), p.379-382
Main Authors: Knowlton, Elizabeth D., Gaffin, Douglas D.
Format: Article
Language:English
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Summary:All scorpions possess jointed, ventral appendages called pectines. These organs have chemosensory, peg-shaped sensilla that detect substrate-borne chemicals. Previous physiological studies show that neurons within peg sensilla respond to an assortment of volatile organic chemical stimulants blown across the sensillar opening. We developed an improved method of chemical stimulant delivery called the mineral oil flood technique to further investigate the neural circuitry of scorpion pectines. The new mineral oil flood technique allows us to deliver chemical stimulants directly to individual sensilla by introducing a polar, liquid substance under non-polar mineral oil. Unlike previous methods of stimulant delivery, the mineral oil flood technique allows for precise control over the duration of direct contact between a liquid stimulant of known concentration and a sensillum.
ISSN:0161-8202
1937-2396
DOI:10.1636/SH08-79SC.1