A high-density genetic map of Sorghum bicolor (L.) Moench based on 2926 AFLP registered , RFLP and SSR markers

Using AFLP technology and a recombinant inbred line population derived from the sorghum cross of BTx623 IS3620C, a high-density genetic map of the sorghum genome was constructed. The 1713cM map encompassed 2926 loci distributed on ten linkage groups; 2454 of those loci are AFLP products generated fr...

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Bibliographic Details
Published in:Plant molecular biology 2002-03, Vol.48 (5-6), p.483-499
Main Authors: Menz, MA, Klein, R R, Mullet, JE, Obert, JA, Unruh, N C, Klein, P E
Format: Article
Language:English
Subjects:
Hordeum vulgare
Oryza sativa
Sorghum
Sorghum bicolor
Zea mays
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Summary:Using AFLP technology and a recombinant inbred line population derived from the sorghum cross of BTx623 IS3620C, a high-density genetic map of the sorghum genome was constructed. The 1713cM map encompassed 2926 loci distributed on ten linkage groups; 2454 of those loci are AFLP products generated from either the EcoRI/Mse delta or PstI/Mse delta enzyme combinations. Among the non-AFLP markers, 136 are SSRs previously mapped in sorghum, and 203 are cDNA and genomic clones from rice, barley, oat, and maize. This latter group of markers has been mapped in various grass species and, as such, can serve as reference markers in comparative mapping. Of the nearly 3000 markers mapped, 692 comprised a LOD greater than or equal to 3.0 framework map on which the remaining markers were placed with lower resolution (LOD
ISSN:0167-4412
1573-5028
DOI:10.1023/A:1014831302392