NIR-labeled nanoparticles engineered for brain targeting: in vivo optical imaging application and fluorescent microscopy evidences

The presence of the blood–brain barrier (BBB) makes extremely difficult to develop efficacious strategies for targeting contrast agents and delivering drugs inside the Central Nervous System (CNS). To overcome this drawback, several kinds of CNS-targeted nanoparticles (NPs) have been developed. In p...

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Bibliographic Details
Published in:Journal of Neural Transmission 2011, Vol.118 (1), p.145-153
Main Authors: Tosi, G., Bondioli, L., Ruozi, B., Badiali, L., Severini, G. M., Biffi, S., De Vita, A., Bortot, B., Dolcetta, D., Forni, F., Vandelli, M. A.
Format: Article
Language:English
Subjects:
Animals
Basic Neurosciences
Blood-Brain Barrier - drug effects
Blood-Brain Barrier - physiology
Brain - anatomy & histology
Brain Chemistry - drug effects
Electrochemistry
Excipients
Female
Fluorescent Dyes
Genetics and Immunology - Original Article
Lactic Acid
Medicine
Medicine & Public Health
Mice
Mice, Inbred BALB C
Microscopy, Confocal
Microscopy, Electron, Scanning
Microscopy, Fluorescence
Nanoparticles
Neurology
Neurosciences
Particle Size
Polyglycolic Acid
Psychiatry
Spectroscopy, Near-Infrared - methods
Tissue Distribution
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Summary:The presence of the blood–brain barrier (BBB) makes extremely difficult to develop efficacious strategies for targeting contrast agents and delivering drugs inside the Central Nervous System (CNS). To overcome this drawback, several kinds of CNS-targeted nanoparticles (NPs) have been developed. In particular, we proposed poly-lactide- co -glycolide (PLGA) NPs engineered with a simil-opioid glycopeptide ( g7 ), which have already proved to be a promising tool for achieving a successful brain targeting after i.v. administration in rats. In order to obtain CNS-targeted NPs to use for in vivo imaging, we synthesized and administrated in mice PLGA NPs with double coverage: near-infrared (NIR) probe (DY-675) and g 7 . The optical imaging clearly showed a brain localization of these novel NPs. Thus, a novel kind of NIR-labeled NPs were obtained, providing a new, in vivo detectable nanotechnology tool. Besides, the confocal and fluorescence microscopy evidences allowed to further confirm the ability of g 7 to promote not only the rat, but also the mouse BBB crossing.
ISSN:0300-9564
1435-1463
DOI:10.1007/s00702-010-0497-1