KRAS Genotyping as Biomarker in Colorectal Cancer: A Comparison of Three Commercial Kits on Histologic Material

The crucial role of KRAS status in new colorectal cancer target therapy raises the issue regarding which testing method to use. This study analysed 112 formalin fixed, paraffin-embedded (FFPE) metastatic tissue samples using three different commercially available kits. A group of 40 KRAS wild-type (...

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Bibliographic Details
Published in:Anticancer research 2010-12, Vol.30 (12), p.5251-5256
Main Authors: CAVALLINI, Aldo, VALENTINI, Anna Maria, LIPPOLIS, Catia, CAMPANELLA, Daniela, GUERRA, Vito, CARUSO, Maria Lucia
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biomarkers, Tumor - genetics
Codon
Colorectal Neoplasms - genetics
Colorectal Neoplasms - pathology
DNA, Neoplasm - analysis
DNA, Neoplasm - genetics
Gastroenterology. Liver. Pancreas. Abdomen
Genes, ras
Genotype
Humans
Medical sciences
Mutation
Neoplasm Metastasis
Paraffin Embedding
Polymerase Chain Reaction - methods
Polymorphism, Restriction Fragment Length
Reagent Strips
Sensitivity and Specificity
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Tumors
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Summary:The crucial role of KRAS status in new colorectal cancer target therapy raises the issue regarding which testing method to use. This study analysed 112 formalin fixed, paraffin-embedded (FFPE) metastatic tissue samples using three different commercially available kits. A group of 40 KRAS wild-type (wt), 40 codon 12-mutated and 32 codon-13 mutated samples, previously evaluated by real-time PCR (TheraScreen kit), used as reference method, were analysed by Ampli-set-K-RAS and K-RAS StripAssay kit (herein called kit A and B, respectively) based on two different technologies. The sensitivity of both kits was 92.5% for wt samples, 100% and 95.0% for kit A and B, respectively for samples mutated in codon 12. The specificity was 100% for both kits for all groups of samples. After a minor modification of the kit A method, its specificity reached 100%. of low cost and easy to use, kit A may be suitable for use in a routine diagnostic setting.
ISSN:0250-7005
1791-7530