Matrix metalloproteinase levels in the drained dialysate reflect the peritoneal solute transport rate: a multicentre study in Japan

• Published in: Nephrology, dialysis, transplantation dialysis, transplantation, 2011-05, Vol.26 (5), p.1695-1701
• Main Authors: HIRAHARA, Ichiro, INOUE, Makoto, UMINO, Tetsuo, SAITO, Osamu, MUTO, Shigeaki, KUSANO, Eiji
• Format: Article
• Language: English
• Subjects: Aged; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Biological and medical sciences; Biomarkers - metabolism; Dialysis Solutions - pharmacokinetics; Emergency and intensive care: renal failure. Dialysis management; Enzyme-Linked Immunosorbent Assay; Female; Follow-Up Studies; Humans; Intensive care medicine; Japan; Kidney Failure, Chronic - enzymology; Kidney Failure, Chronic - therapy; Male; Matrix Metalloproteinase 2 - metabolism; Medical sciences; Middle Aged; Nephrology. Urinary tract diseases; Nephropathies. Renovascular diseases. Renal failure; Peritoneal Dialysis - adverse effects; Peritoneum - enzymology; Peritoneum - injuries; Peritoneum - pathology; Prognosis; Renal failure; Tissue Inhibitor of Metalloproteinase-1 - metabolism
• ISSN: 0931-0509; 1460-2385
• DOI: 10.1093/ndt/gfq593

Long-term peritoneal dialysis (PD) leads to peritoneal injury with high solute transport of the peritoneal membrane. At worst, peritoneal injury leads to encapsulating peritoneal sclerosis with an extremely high mortality rate. To perform PD safely and adequately, it is necessary to monitor peritoneal injury. The aim of this study was to investigate the potential of matrix metalloproteinases (MMPs) as new indicators of peritoneal injury. The subjects included 215 PD patients with end-stage renal disease at 20 centres in Japan. MMPs or tissue inhibitors of MMP (TIMPs) in the drained dialysate were quantified with enzyme-linked immunosorbent assay. The peritoneal solute transport rate was assessed to estimate peritoneal injury and PD efficiency by the peritoneal equilibration test (PET). MMP-2, MMP-3 and TIMP-1 levels in the drained dialysate obtained by the PET were correlated with the D/P Cr ratios (ρ = 0.69, ρ = 0.52, ρ = 0.55, respectively) and the D/D0 glucose ratios (ρ = -0.60, ρ = -0.47, ρ = -0.48, respectively). The measured D/S ratios of MMP-2 and TIMP-1 were significantly higher than the expected D/S ratios when MMP-2 and TIMP-1 would have been transported from only the circulation. The measured D/S ratios of MMP-3 nearly corresponded to the expected ratios. MMP-1 and TIMP-2 in the drainage were undetected in most patients. From these results, most MMP-2 in the drained dialysate may be produced from the peritoneum, and MMP-2 is expected to be a useful marker of peritoneal injury or change in peritoneal solute transport.