Making and Breaking Peptide Bonds: Protein Engineering Using Sortase

Sortases are a class of bacterial enzymes that possess transpeptidase activity. It is their ability to site‐specifically break a peptide bond and then reform a new bond with an incoming nucleophile that makes sortase an attractive tool for protein engineering. This technique has been adopted for a r...

Full description

Saved in:
Bibliographic Details
Published in:Angewandte Chemie International Edition 2011-05, Vol.50 (22), p.5024-5032
Main Authors: Popp, Maximilian Wei-Lin, Ploegh, Hidde L.
Format: Article
Language:English
Subjects:
Amino Acid Motifs
Aminoacyltransferases - chemistry
Aminoacyltransferases - genetics
Aminoacyltransferases - metabolism
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Cysteine Endopeptidases - chemistry
Cysteine Endopeptidases - genetics
Cysteine Endopeptidases - metabolism
Enzymes
Protein Engineering
Protein folding
protein modifications
Protein Structure, Tertiary
Recombinant Fusion Proteins - chemistry
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
site specificity
sortase
Staphylococcus aureus - enzymology
transpeptidation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Sortases are a class of bacterial enzymes that possess transpeptidase activity. It is their ability to site‐specifically break a peptide bond and then reform a new bond with an incoming nucleophile that makes sortase an attractive tool for protein engineering. This technique has been adopted for a range of applications, from chemistry‐based to cell biology and technology. In this Minireview we provide a brief overview of the biology of sortase enzymes and current applications in protein engineering. We identify areas that lend themselves to further innovation and that suggest new applications. It takes all sortases: Enzymatic formation of a peptide bond using the sortase A transpeptidase (SrtA) provides a convenient and mild means for engineering proteins to contain nongenetically templated modifications. Myriad applications are possible, from producing homogeneous post‐translational modification mimics, assembling protein domains, to anchoring proteins to solid surfaces.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201008267