Fungal bioremediation of the creosote-contaminated soil: Influence of Pleurotus ostreatus and Irpex lacteus on polycyclic aromatic hydrocarbons removal and soil microbial community composition in the laboratory-scale study

• Published in: Chemosphere (Oxford) 2008-11, Vol.73 (9), p.1518-1523
• Main Authors: Byss, Marius, Elhottová, Dana, Tříska, Jan, Baldrian, Petr
• Format: Article
• Language: English
• Subjects: Actinobacteria; Basidiomycetes; Irpex lacteus; PAH; Pleurotus ostreatus; PLFA; Pseudomonas; Soil microbial community; Wood-rotting basidiomycetes
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2008.07.030

The aim of this study was to determine the efficacy of selected basidiomycetes in the removing of polycyclic aromatic hydrocarbons (PAH) from the creosote-contaminated soil. Fungi Pleurotus ostreatus and Irpex lacteus were supplemented with creosote-contaminated (50–200 mg kg −1 PAH) soil originating from a wood-preserving plant and incubated at 15 °C for 120 d. Either fungus degraded PAH with 4–6 aromatic rings more efficiently than the microbial community present initially in the soil. PAH removal was higher in P. ostreatus treatments (55–67%) than in I. lacteus treatments (27–36%) in general. P. ostreatus (respectively, I. lacteus) removed 86–96% (47–59%) of 2-rings PAH, 63–72% (33–45%) of 3-rings PAH, 32–49% (9–14%) of 4-rings PAH and 31–38% (11–13%) of 5–6-rings PAH. MIS (Microbial Identification System) Sherlock analysis of the bacterial community determined the presence of dominant Gram-negative bacteria (G−) Pseudomonas in the inoculated soil before the application of fungi. Complex soil microbial community was characterized by phospholipid fatty acids analysis followed by GC–MS/MS. Either fungus induced the decrease of bacterial biomass (G− bacteria in particular), but the soil microbial community was influenced by P. ostreatus in a different way than by I. lacteus. The bacterial community was stressed more by the presence of I. lacteus than P. ostreatus (as proved by the ratio of the fungal/bacterial markers and by the ratio of trans/ cis mono-unsaturated fatty acids). Moreover, P. ostreatus stimulated the growth of Gram-positive bacteria (G+), especially actinobacteria and these results indicate the potential of the positive synergistic interaction of this fungus and actinobacteria in creosote biodegradation.