Efficacy of Polyvalent Bacteriophage P-27/HP to Control Multidrug Resistant Staphylococcus aureus Associated with Human Infections

Emergence of multidrug resistant strains has created serious problem for safe eradication of Staphylococcus aureus infections. Therefore, there is an urgent need to develop novel antibacterial agents to control this pathogen. Bacteriophages kill bacteria irrespective of their antibiotic sensitivity...

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Bibliographic Details
Published in:Current microbiology 2011, Vol.62 (1), p.255-260
Main Authors: Gupta, Ragini, Prasad, Yogendra
Format: Article
Language:English
Subjects:
Animals
Antibacterial agents
Bacteremia - prevention & control
Bacteria
Biomedical and Life Sciences
Biotechnology
Colony Count, Microbial
Disease Models, Animal
Drug resistance
Drug Resistance, Multiple, Bacterial
Humans
Injections, Subcutaneous
Life Sciences
Mice
Microbial Viability
Microbiology
Sewage
Sewage - virology
Spleen - microbiology
Staphylococcal Infections - therapy
Staphylococcus aureus
Staphylococcus aureus - drug effects
Staphylococcus aureus - virology
Staphylococcus infections
Staphylococcus Phages - growth & development
Staphylococcus Phages - isolation & purification
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Summary:Emergence of multidrug resistant strains has created serious problem for safe eradication of Staphylococcus aureus infections. Therefore, there is an urgent need to develop novel antibacterial agents to control this pathogen. Bacteriophages kill bacteria irrespective of their antibiotic sensitivity and thus they can be used as potent prophylactic/therapeutic agent to treat such infections. Here, we report isolation of broad host range anti-staphylococcal lytic bacteriophage P-27/HP from sewage water. This phage was able to inhibit 17 of 28 (60%) human disease associated S. aureus isolates. In vitro studies revealed its strong lytic efficacy to diminish S. aureus 27/HP population (c.f.u.) by more than 5.0 logs (P < 0.0001) (equivalent to 99.99%) in 3 h at 0.01 MOI. In vivo lytic efficacy analysis showed that a single subcutaneous injection of phage P-27/HP (10⁷ p.f.u.) was sufficient to protect S. aureus 27/HP infected (5 × 10⁸ c.f.u.) mice from bacteremia and subsequent death. A considerable decline of more than 6 logs (99.9%) in splenic S. aureus 27/HP c.f.u. count was noted at the 3 days of phage treatment. In conclusion, our results suggest that phage P-27/HP is polyvalent in nature and has high-lytic potential towards S. aureus, thus, a therapy employing this phage would be efficacious to control S. aureus infections.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-010-9699-x