Investigation of cytotoxic T-lymphocyte-associated protein 4 gene polymorphisms in symptomatic gallstone disease

Abstract Gallstone disease (GSD), which is increasingly prevalent in Taiwan, develops through a complex process involving genetic, environmental, and immune factors. Cytotoxic T-lymphocyte-associated protein 4 (CTLA4) limits T-cell proliferation. The present study looked for associations between sym...

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Bibliographic Details
Published in:Human immunology 2011-04, Vol.72 (4), p.355-358
Main Authors: Shih, Shou-Chuan, Yang, Horng-Woei, Chang, Tzu-Yang, Hu, Kuang-Chun, Chang, Shih-Chuan, Lin, Chiung-Ling, Hung, Chien-Yuan, Wang, Horng-Yuan, Lin, Marie, Lee, Yann-Jinn
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Alleles
Allergy and Immunology
Antigens, CD - genetics
Case-Control Studies
Case–control study
Computer programs
Cross-Sectional Studies
CTLA-4 Antigen
CTLA-4 protein
CTLA4
Cytotoxicity
Data processing
Enumeration
Female
Gallstone disease
Gallstones - genetics
Gene Frequency
Gene polymorphism
Genetic Predisposition to Disease
Genotype
Genotyping
Haplotypes
Humans
Lymphocytes T
Male
Middle Aged
Polymorphism, Single Nucleotide - genetics
Single nucleotide polymorphism
software
Taiwan
Taiwanese
Young Adult
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Summary:Abstract Gallstone disease (GSD), which is increasingly prevalent in Taiwan, develops through a complex process involving genetic, environmental, and immune factors. Cytotoxic T-lymphocyte-associated protein 4 (CTLA4) limits T-cell proliferation. The present study looked for associations between symptomatic GSD and polymorphisms of the CTLA4 gene. For this case–control cross-sectional study among Taiwanese, 275 patients with symptomatic GSD and 852 controls were enrolled. Genotyping of CTLA4−318 C/T , +49 A/G , and CT60 A/G single nucleotide polymorphisms (SNPs) was performed by polymerase chain reaction–restriction fragment length polymorphism. The genotype, allele, carrier, and haplotype frequencies were calculated by direct counting or with Haploview 4.1 software. Genotype, allele, carrier, and haplotype frequencies of the CTLA4 SNPs studied were equally distributed in symptomatic GSD patients and controls. No significant associations between symptomatic GSD and these 3 SNPs were observed. Our data suggest that CTLA4−318 C/T , +49 A/G , and CT60 A/G SNPs do not confer increased susceptibility to symptomatic GSD.
ISSN:0198-8859
1879-1166
DOI:10.1016/j.humimm.2011.01.004