Deciphering cellular functions of protein phosphatases by comparison of gene expression profiles in Saccharomyces cerevisiae

• Published in: Journal of bioscience and bioengineering 2010-05, Vol.109 (5), p.433-441
• Main Authors: Hirasaki, Masataka, Nakamura, Fumika, Yamagishi, Kazuo, Numamoto, Minori, Shimada, Yukiko, Uehashi, Keigo, Muta, Shigeru, Sugiyama, Minetaka, Kaneko, Yoshinobu, Kuhara, Satoru, Harashima, Satoshi
• Format: Article
• Language: English
• Subjects: ANTIBIOTICOS; ANTIBIOTICS; ANTIBIOTIQUE; Biological and medical sciences; Biotechnology; Calcofluor white; Congo red; Data processing; EXPRESION GENICA; EXPRESSION DES GENES; Expression profile; FENOTIPOS; FONCTION PHYSIOLOGIQUE; FOSFATOS; FUNCION FISIOLOGICA; Fundamental and applied biological sciences. Psychology; Gene deletion; GENE EXPRESSION; Gene Expression Profiling - methods; Gene Expression Regulation, Enzymologic - physiology; Gene Expression Regulation, Fungal - physiology; Gene Knockout Techniques; Glycerol; http://www.fao.org/aos/agrovoc#c_27527; http://www.fao.org/aos/agrovoc#c_35986; http://www.fao.org/aos/agrovoc#c_492; http://www.fao.org/aos/agrovoc#c_5013; http://www.fao.org/aos/agrovoc#c_5776; http://www.fao.org/aos/agrovoc#c_5837; http://www.fao.org/aos/agrovoc#c_6724; Hygromycin B; MUTANT; MUTANTES; MUTANTS; Mutation - genetics; Pearson's correlation; PHENOTYPE; PHENOTYPES; Phenotypic analysis; PHOSPHATE; PHOSPHATES; Phosphoric Monoester Hydrolases - metabolism; PHYSIOLOGICAL FUNCTIONS; Protein phosphatase; SACCHAROMYCES CEREVISIAE; Saccharomyces cerevisiae - classification; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1016/j.jbiosc.2009.10.023

Expression profiles of protein phosphatase (PPase) disruptants were analyzed by use of Pearson's correlation coefficient to find profiles that correlated with those of 316 Reference Gene ( RG) disruptants harboring deletions in genes with known functions. Twenty-six Δppase disruptants exhibited either a positive or negative correlation with 94 RG disruptants when the p value for Pearson's correlation coefficient was > 0.2. Some of the predictions that arose from this analysis were tested experimentally and several new Δppase phenotypes were found. Notably, Δsit4 and Δsiw14 disruptants exhibited hygromycin B sensitivity, Δsit4 and Δptc1 disruptants grew slowly on glycerol medium, the Δptc1 disruptant was found to be sensitive to calcofluor white and congo red, while the Δppg1 disruptant was found to be sensitive to congo red. Because on-going analysis of expression profiles of Saccharomyces cerevisiae disruptants is rapidly generating new data, we suggest that the approach used in the present study to explore PPase function is also applicable to other genes.