Correlation between molecular markers and adaptively significant genetic variation in Bromus tectorum (Poaceae), an inbreedingannual grass

Single sequence repeat (SSR) and amplified fragment length polymorphic (AFLP) molecular marker genotypes in cheatgrass (Bromus tectorum) were compared to published data on phenotypic variation in seed dormancy, vernalization requirement, and resistance to the pathogen Ustilago bullata. Several featu...

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Bibliographic Details
Published in:American journal of botany 2004-06, Vol.91 (6), p.797-803
Main Authors: Ramakrishnan, Alisa P, Meyer, Susan E, Waters, Jennifer, Stevens, Mikel R, Coleman, Craig E, Fairbanks, Daniel J
Format: Article
Language:English
Subjects:
AFLP
Botany
Bromus tectorum
cheatgrass
Genetic diversity
Genetic markers
genetic variation
Genotype & phenotype
Grasses
microsatellite
molecular marker
Pathogens
phenotypic variation
Population genetics
SSR
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Summary:Single sequence repeat (SSR) and amplified fragment length polymorphic (AFLP) molecular marker genotypes in cheatgrass (Bromus tectorum) were compared to published data on phenotypic variation in seed dormancy, vernalization requirement, and resistance to the pathogen Ustilago bullata. Several features of cheatgrass facilitated this study: it is a recent invader in the western United States, has considerable phenotypic polymorphism, and is an obligate self‐pollinator. Forty self‐pollinating lines from four populations common to the three phenotypic data sets were analyzed for molecular genetic variation using seven SSR loci and 31 AFLP loci. We examined correlations between distance matrices using the Mantel test for each pair of studies. The two molecular data sets were significantly correlated (r = 0.636). The AFLP markers often distinguished among several lines with identical SSR genotypes. The AFLP data were also significantly correlated with the phenotypic data (r values from 0.4640 to 0.5658), but the SSR data were much more highly correlated (r values from 0.677 to 0.844). The difference between molecular marker systems was especially notable when an outlier population from Potosi Pass, Nevada, was excluded from the analysis. These results suggest that SSR markers may be good surrogates for phenotypic traits in population genetic studies of strongly inbreeding species such as cheatgrass.
ISSN:0002-9122
1537-2197
DOI:10.3732/ajb.91.6.797