Inhibition of peptide deformylase in Nicotiana tabacum leads to decreased D1 protein accumulation, ultimately resulting in a reduction of photosystem II complexes

Eukaryotic homologs of bacterial peptide deformylases were recently found in several vascular plants and may be essential in chloroplast protein processing. Treating tobacco seedlings with the peptide deformylase inhibitor actinonin resulted in leaf chlorosis and reduced growth and development, indi...

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Bibliographic Details
Published in:American journal of botany 2004-09, Vol.91 (9), p.1304-1311
Main Authors: Hou, Cai-Xia, Dirk, Lynnette M. A, Williams, Mark A
Format: Article
Language:English
Subjects:
actinonin
Biochemistry
Botany
Chloroplasts
D1 protein
Leaves
Monomers
Nicotiana tabacum
peptide deformylase
Peptides
photosynthesis
Photosystem II
Physiology and Development
Plants
Polysorbates
Proteins
PSII complex
Seedlings
Thylakoids
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Summary:Eukaryotic homologs of bacterial peptide deformylases were recently found in several vascular plants and may be essential in chloroplast protein processing. Treating tobacco seedlings with the peptide deformylase inhibitor actinonin resulted in leaf chlorosis and reduced growth and development, indicative of a systemic movement of the inhibitor. Photosystem II (PSII) activity was reduced, manifested as a significant decrease in the maximum quantum efficiency of photosystem II. Accumulation and assembly of nascent Dl protein into PSII monomers was also reduced, eventually leading to PSII disassembly and leaf necrosis. Processing and assembly of D1 protein in tobacco was a major and potentially critical target of peptide deformylase inhibition. These results confirm that N-terminal deformylation is an essential step in the accumulation and assembly of PSII subunit polypeptides in the chloroplasts of vascular plants.
ISSN:0002-9122
1537-2197
DOI:10.3732/ajb.91.9.1304