Detection of staphylococcal genes directly from cerebrospinal and peritoneal fluid samples using a multiplex polymerase chain reaction

A multiplex polymerase chain reaction (PCR) that can simultaneously detect eubacterial isolates and the methicillin-susceptibility of staphylococcal isolates from cerebrospinal and peritoneal fluid samples was compared to conventional microbiological methods. Using conventional methods, bacteria wer...

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Bibliographic Details
Published in:European journal of clinical microbiology & infectious diseases 1998-04, Vol.17 (4), p.272-274
Main Authors: SCHMITZ, F.-J, STEIERT, M, HOFMANN, B, FLUIT, A. C, VERHOEF, J, HEINZ, H.-P, JONES, M. E
Format: Article
Language:English
Subjects:
Ascitic Fluid - microbiology
Bacteria
Bacterial diseases
Bacterial diseases of the nervous system. Bacterial myositis
Biological and medical sciences
Cerebrospinal Fluid - microbiology
Eubacteria
Genes, Bacterial - genetics
Human bacterial diseases
Humans
Infectious diseases
Medical sciences
Microbiology
Polymerase Chain Reaction
Staphylococcal Infections - cerebrospinal fluid
Staphylococcal Infections - diagnosis
Staphylococcus aureus - genetics
Staphylococcus epidermidis - genetics
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Summary:A multiplex polymerase chain reaction (PCR) that can simultaneously detect eubacterial isolates and the methicillin-susceptibility of staphylococcal isolates from cerebrospinal and peritoneal fluid samples was compared to conventional microbiological methods. Using conventional methods, bacteria were isolated from 8% (29/350) of the cerebrospinal fluid samples and from 5% (3/60) of the peritoneal fluid samples. All isolates except two Staphylococcus epidermidis isolates were also detected using the multiplex PCR. Coagulase-negative staphylococci and Staphylococcus aureus were correctly identified using both methods. The multiplex PCR can rapidly and simultaneously detect eubacteria, and the methicillin susceptibility of staphylococci from samples containing > or = 10(2) cfu/ml of bacteria.
ISSN:0934-9723
1435-4373
DOI:10.1007/BF01699985