Brainstem Hap1–Ahi1 is involved in insulin-mediated feeding control

The function of the brainstem Hap1–Ahi1 complex in the regulation of feeding behavior was investigated. When mice were fasted or treated with 2-deoxy-d-glucose (2-DG), Hap1–Ahi1 was significantly upregulated. By using streptozotocin (STZ) to decrease the circulating insulin in mice, Hap1–Ahi1 was si...

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Bibliographic Details
Published in:FEBS letters 2011-01, Vol.585 (1), p.85-91
Main Authors: Niu, Shao-Na, Huang, Zhen-Bo, Wang, Hao, Rao, Xiu-Rong, Kong, Hui, Xu, Jing, Li, Xiao-Jiang, Yang, Chuan, Sheng, Guo-Qing
Format: Article
Language:English
Subjects:
2-deoxyglucose
Abelson helper integration site 1
Animals
Blood Glucose - metabolism
Blotting, Western
body weight
brain stem
Brain Stem - drug effects
Brain Stem - metabolism
Brainstem
Cell Line, Tumor
Deoxyglucose - pharmacology
Diabetes Mellitus, Experimental - blood
Diabetes Mellitus, Experimental - genetics
Diabetes Mellitus, Experimental - metabolism
Eating - drug effects
Energy homeostasis
Fasting
feeding behavior
Food intake
Gene Expression Regulation - drug effects
Huntingtin-associated protein 1
Hypoglycemic Agents - pharmacology
Immunohistochemistry
insulin
Insulin - pharmacology
Male
Mice
Mice, Inbred C57BL
Multiprotein Complexes - metabolism
Nerve Tissue Proteins - genetics
Nerve Tissue Proteins - metabolism
Nucleus of the solitary tract
Protein Binding
protein synthesis
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA Interference
signal transduction
Solitary Nucleus - metabolism
streptozotocin
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Summary:The function of the brainstem Hap1–Ahi1 complex in the regulation of feeding behavior was investigated. When mice were fasted or treated with 2-deoxy-d-glucose (2-DG), Hap1–Ahi1 was significantly upregulated. By using streptozotocin (STZ) to decrease the circulating insulin in mice, Hap1–Ahi1 was significantly increased. Furthermore, intra-brain injection of insulin decreased the expression of Hap1–Ahi1 in the brainstem. Moreover, when we knocked down the expression of brainstem Hap1 by RNAi, the mice showed decreased food intake and lower body weights. Collectively, our results indicate that the Hap1–Ahi1 complex in the brainstem works as a sensor for insulin signals in feeding control. Ahi1physically interacts with Hap1: shown by anti bait coimmunoprecipitation (view interactions 1, 2)
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2010.11.059