Phylogenetic relationships among Saccharum clones in Pakistan revealed by RAPD markers

• Published in: Genetics and molecular research 2010-01, Vol.9 (3), p.1673-1682
• Main Authors: Nawaz, S, Khan, F A, Tabasum, S, Zakria, M, Saeed, A, Iqbal, M Z
• Format: Article
• Language: English
• Subjects: DNA, Plant - genetics; Genetic Variation - genetics; Genotype; Pakistan; Phylogeny; Polymerase Chain Reaction; Random Amplified Polymorphic DNA Technique - methods; Saccharum; Saccharum - classification; Saccharum - genetics; Saccharum officinarum
• ISSN: 1676-5680; 1676-5680
• DOI: 10.4238/vol9-3gmr903

Forty sugarcane genotypes (clones), including elite lines, commercial cultivars of Saccharum officinarum and S. barberi clones, were fingerprinted with 30 RAPD markers, using a PCR-based marker assay. The genetic distance for RAPD data was determined according to Nei, and relationships between accessions were graphed in a dendrogram. Genetic distance values ranging from 16.2 to 86.3% were observed among the 40 sugarcane accessions. The lowest genetic distance was found between genotypes US-406 and US-186. These two genotypes differed from each other in only 25 bands with 15 different primers. Genotypes Col-54 and CP-72-2086 were the second most similar group, with a genetic distance of 19.46%. The most dissimilar of all the accessions were CP-77-400 and US-133, with a genetic distance of 86.3%. RAPD fingerprints help sugarcane breeders clarify the genetic pedigree of commercial sugarcane varieties and can be used to evaluate the efficiency of conventional breeding methods.