Population genetic analysis of European Prunus spinosa (Rosaceae) using chloroplast DNA markers

Chloroplast DNA diversity in Prunus spinosa, a common shrub of European deciduous forests, was assessed using the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) technique. Thirty-two haplotypes were detected in 25 populations spread across the European continent. Ten h...

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Bibliographic Details
Published in:American journal of botany 2002-08, Vol.89 (8), p.1223-1228
Main Authors: Mohanty, Aparajita, Martín, Juan Pedro, Aguinagalde, Itziar
Format: Article
Language:English
Subjects:
Biological taxonomies
Botany
CAPS
Chloroplast DNA
Chloroplasts
cpDNA diversity
Deciduous forests
Deoxyribonucleic acid
DNA
Ecological genetics
Europe
Flowers & plants
Haplotypes
Microsatellites
PCR‐RFLP
phylogeography
Plants
Population Biology
Population genetics
private haplotypes
Prunus spinosa
Rosaceae
Species diversity
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Summary:Chloroplast DNA diversity in Prunus spinosa, a common shrub of European deciduous forests, was assessed using the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) technique. Thirty-two haplotypes were detected in 25 populations spread across the European continent. Ten haplotypes were shared by two or more populations, and 22 were private. The major proportion of the total cpDNA diversity ($H_T = 0.73$) was located within the populations ($H_S = 0.49$), and differentiation between populations was low ($G_{ST} = 0.33$) compared with other forest species. Haplotype diversity was higher in southern Europe than in northern Europe, indicating probable localization of glacial refugia in southern Europe. The minimum-length spanning tree of haplotypes showed incongruency between the phylogeny of haplotypes and their geographic locations. This might be the result of intensive seed movements following recolonization, which thereby erased the phylogeographic structure in P. spinosa.
ISSN:0002-9122
1537-2197
DOI:10.3732/ajb.89.8.1223