The effect of a low concentration of hypochlorous acid on rhinovirus infection of nasal epithelial cells

Low concentrations of hypochlorous acid (HOCl) have been shown to exhibit both antibacterial and anti-influenza virus activity, but HOCl still has not been used to kill human rhinovirus (HRV). To model the antiviral effect of nasal irrigation with low-level HOCl in patients with the common cold, we...

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Bibliographic Details
Published in:American journal of rhinology & allergy 2011-01, Vol.25 (1), p.40-44
Main Authors: Yu, Myeong Sang, Park, Hyung Wook, Kwon, Hyun Ja, Jang, Yong Ju
Format: Article
Language:English
Subjects:
Cell Membrane Permeability - drug effects
Cells, Cultured
Electric Impedance
Humans
Hypochlorous Acid - pharmacology
Interleukin-6 - genetics
Interleukin-6 - immunology
Interleukin-6 - metabolism
Interleukin-8 - genetics
Interleukin-8 - immunology
Interleukin-8 - metabolism
Nasal Mucosa - drug effects
Nasal Mucosa - immunology
Nasal Mucosa - metabolism
Nasal Mucosa - pathology
Nasal Mucosa - virology
Picornaviridae Infections - drug therapy
Picornaviridae Infections - immunology
Picornaviridae Infections - pathology
Primary Cell Culture
Rhinovirus - pathogenicity
Rhinovirus - physiology
Viral Load - drug effects
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Summary:Low concentrations of hypochlorous acid (HOCl) have been shown to exhibit both antibacterial and anti-influenza virus activity, but HOCl still has not been used to kill human rhinovirus (HRV). To model the antiviral effect of nasal irrigation with low-level HOCl in patients with the common cold, we tested the effects of a low concentration of HOCl on HRV infection of primary human nasal epithelial cells (HNEC). Cells were infected with HRV for 24 hours and treated with HOCl three times, for 5 minutes each time, at 12 hour intervals. The effects of HOCl on rhinovirus-induced secretion of IL-6 and IL-8 were assessed by ELISA and HRV replication was determined by viral titration. HOCl treatment significantly inhibited HRV-induced secretion of IL-6 and IL-8 and significantly reduced viral titer. The effects of HOCl peaked at 1 minute after HOCl generation and decreased thereafter. These in vitro findings indicate that nasal irrigation with low-level HOCl solution may improve clinical symptoms in patients with the common cold.
ISSN:1945-8924
1945-8932
DOI:10.2500/ajra.2011.25.3545