Periplaneta americana midgut proteases differentially expressed against dietary components from different plant seeds

Insect midgut proteases are known to be regulated by plant protease inhibitors. In the present study, the antinutritional effects of a variety of seed extracts against Periplaneta americana (Linnaeus) (Dictyoptera: Blattidae) midgut proteases are assessed in vitro and in vivo. Bioassays are conducte...

Full description

Saved in:
Bibliographic Details
Published in:Physiological entomology 2011-06, Vol.36 (2), p.180-186
Main Authors: HIVRALE, VANDANA K, LOMATE, PURUSHOTTAM R, KALVE, NEETA D, KACHOLE, MANVENDRA S
Format: Article
Language:English
Subjects:
Bioassay
bioassays
Blattidae
Crops
Datura
Datura metel
Diets
electrophoresis
gel-X-ray film contact print method
gene expression regulation
Glycine max
insensitive proteases
Midgut
Mobility
Mucuna pruriens
Periplaneta americana
Pests
physiology
Plant extracts
plant protease inhibitors
Powder
powders
Proteinase inhibitors
proteinases
Proteolysis
Ricinus communis
Sapindus
Sapindus trifoliatus
seed extracts
Seeds
Survival
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Insect midgut proteases are known to be regulated by plant protease inhibitors. In the present study, the antinutritional effects of a variety of seed extracts against Periplaneta americana (Linnaeus) (Dictyoptera: Blattidae) midgut proteases are assessed in vitro and in vivo. Bioassays are conducted by allowing P. americana to feed on diets incorporated with the tested seed extracts. Low survival rates are recorded on a diet incorporated with seed powders of Ricinus communis (10%), Glycine max (30%), Datura alba (50%) and Mucuna pruriens (50%). Proteolytic and residual proteolytic activities are highly inhibited by the four seed extracts. Electrophoretic analysis shows that the majority of P. americana midgut protease isoforms are inhibited by the four seed extracts in vivo as well as in vitro. The midgut physiology of P. americana is affected by the seed extracts of D. alba, M. pruriens and Sapindus laurifolius, by over synthesising or changing mobilities of existing protease isoforms. Furthermore, some key proteases in P. americana midgut are involved in the regulation of other protease isoforms. These results suggest that seed extracts from the above plants are potential sources of plant protease inhibitors for managing economically important crop pests.
ISSN:0307-6962
1365-3032
DOI:10.1111/j.1365-3032.2011.00784.x