A Babesia bovis gene syntenic to Theileria parva p67 is expressed in blood and tick stage parasites

• Published in: Veterinary parasitology 2010-10, Vol.173 (3), p.211-218
• Main Authors: Freeman, Jeanne M., Kappmeyer, Lowell S., Ueti, Massaro W., McElwain, Terry F., Baszler, Timothy V., Echaide, Ignacio, Nene, Vishvanath M., Knowles, Donald P.
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; amino acid sequences; Animals; Babesia bigemina; Babesia bovis; Babesia bovis - genetics; Babesia bovis - immunology; Babesia equi; Base Sequence; Boophilus microplus; Comparative genomics; conserved sequences; Female; Fluorescent Antibody Technique, Indirect; gene expression regulation; Ixodidae; Mice; Mice, Inbred BALB C; Molecular Sequence Data; monoclonal antibodies; p67; Piroplasmia; Protozoan Vaccines - genetics; Protozoan Vaccines - immunology; Recombinant Proteins - genetics; Recombinant Proteins - immunology; Reverse Transcriptase Polymerase Chain Reaction - veterinary; RNA, Protozoan - chemistry; RNA, Protozoan - genetics; Sequence Alignment; Sequence Analysis, DNA; Synteny; Synteny - genetics; Theileria equi; Theileria parva; Theileria parva - genetics; Theileria parva - immunology; theileriosis; ticks; Ticks - parasitology
• ISSN: 0304-4017; 1873-2550
• DOI: 10.1016/j.vetpar.2010.06.024

Completion of the Babesia bovis (T2Bo strain) genome provides detailed data concerning the predicted proteome of this parasite, and allows for a bioinformatics approach to gene discovery. Comparative genomics of the hemoprotozoan parasites B. bovis and Theileria parva revealed a highly conserved syntenic block of genes flanking the p67 gene of T. parva, a sporozoite stage-specific vaccine candidate against East Coast fever (ECF). The syntenic gene in B. bovis, designated bov57, encodes a protein of limited amino acid sequence identity (11.8%) to p67. Monoclonal antibodies were produced against recombinant BOV57 and were used to demonstrate expression of BOV57 in merozoite and kinete stages of the T2Bo strain of B. bovis. Transcript levels of bov57 in kinetes were increased 100-fold in comparison to msa-1, a previously identified gene encoding an erythrocyte stage surface protein. Amino acid sequence comparisons between the T2Bo strain and two attenuated and virulent strains from Argentina and Australia revealed a high degree of sequence conservation in BOV57 among these geographically and pathogenically divergent isolates (97% amino acid sequence identity). Additional genomic comparisons show that the bov57 gene locus is also conserved in Babesia bigemina and Babesia equi. While not identifiable through amino acid or nucleotide sequence similarity, the conserved gene order within this locus in multiple piroplasms may suggest a critical function adapted for each species’ unique host and life-cycle.