Evaluation of Metarhizium anisopliae strains as potential biocontrol agents of the tick Rhipicephalus (Boophilus) microplus and the cotton stainer Dysdercus peruvianus

• Published in: Fungal ecology 2008-05, Vol.1 (2), p.78-88
• Main Authors: Lubeck, Irina, Arruda, Walquíria, Souza, Bárbara K., Stanisçuaski, Fernanda, Carlini, Célia R., Schrank, Augusto, Vainstein, Marilene H.
• Format: Article
• Language: English
• Subjects: Arthropoda; Biological control; Boophilus microplus; Chitinases; Dysdercus peruvianus; Ixodidae; Metarhizium anisopliae; Rhipicephalus; Rhipicephalus (Boophilus) microplus
• ISSN: 1754-5048
• DOI: 10.1016/j.funeco.2008.09.002

The negative aspects of traditional pest control have led to the investigation of alternative methods such as biological control. Metarhizium anisopliae, well known as an entomopathogenic fungus capable of actively invading and killing its hosts and thus a candidate biopesticide, is here tested against two agricultural pests of economic/social importance and also evaluated for its chitinolytic secretion and capacity to grow and sporulate at different temperatures. None of the isolates was able to grow below 4 °C or above 37 °C. Chitinolytic activity under artificial growth conditions revealed that Rhipicephalus ( Boophilus) microplus cuticle induces N-acetyl-β- d-glucosaminidase and endochitinase activities more efficiently than Dysdercus peruvianus cuticle and that glucose did not repress those activities. Bioassays were carried out with R. microplus females and fourth instar D. peruvianus. Six isolates of M. anisopliae were pathogenic to the engorged female cattle ticks. E6, GC47 and CG97 were the most virulent isolates for both arthropod models although differences were seen among them. M. anisopliae strains caused 90–100 % mortality on the fourth post-infection day in R. microplus. D. peruvianus females were more sensitive to fungal infection than males, and the most virulent strains caused 50 % mortality on the third to fourth day post-infection. Our studies suggest that M. anisopliae strain CG47 is a candidate for commercial pesticide formulations due to its capacity to kill both hosts and its ability to sporulate at higher temperatures.